Evaluation of TUBB۳, PSMB۷, CBFB, and COL۹A۱ genes involved in the development of Squamous Cell Lung Cancer (SCC)

Squamous Cell Lung Cancer (SCC) is considered one of the types of lung cancer, and this type is still the deadliest cancer in the United States and around the world. Numerous genes are involved in the development of this type of cancer. Detection of such genes can be a suitable tool for the diagnosis and treatment of squamous cell lung cancer in the future. The aim of this study was to identify the main genes (hub genes) related to SCC, whose expression can be related to the death rate of patients. Materials and Methods:First, to study the main genes related to SCC, RNAseq data available in the TCGA database was used. Genes with significant expression changes (FDR<۰.۰۱, logFC>۱) and associated with poor prognosis were selected. Enrichr database and MSigdb database were used to identify pathways related to candidate genes. To detect the hub genes, the Protein-Protein Interaction (PPI) network was used and those with the highest degree were selected among the candidate genes. The linear model method was used to investigate the difference in expression and the Cox regression test was used to investigate the relationship between gene expression and mortality rate. All pre-processing and analysis were done using R software.Results:The expression difference findings showed ۱۸۸۳ genes had significantly increased expression in cancer samples compared to normal. Instead, ۱۴۲۱ genes were identified whose increased expression was associated with poor prognosis (HR>۱, logRank<۰.۰۵). The results showed ۱۷۵ genes are both significantly increased and associated with poor prognosis. The results for ۱۷۵ identified genes showed that these genes are involved in the pathways of metastasis, glycolysis, and DNA repair.Conclusion:This study suggests that TUBB۳, PSMB۷, CBFB, and COL۹A۱ genes play an important role in the pathogenesis of SCC and can be suitable diagnostic and therapeutic targets.

کلیدواژه ها:

Squamous Cell Lung Cancer (SCC) ، TCGA ، Enrichr ، Hub Genes ، Msigdb