The effect of N۲O gas on serum cobalamin and homocysteine in patients undergoing orthopedic surgery

Nitrous oxide (N۲O) is an inhaled anesthetic drug that uses during surgery and recently proposed as a treatment for mood disorder in a research setting. Exposure to N۲O during surgery can cause hematopoietic and neural side effects. The purpose of this study was to the designation of serum cobalamin, homocysteine and CBC factors changes after exposure to nitrous oxide during orthopedic surgery. This study has been done on ۳۰ patients undergoing orthopedic operations from ۲۰۱۶ until ۲۰۱۸. All patients are scheduled for orthopedic surgery. All patients received nitrous oxide during their anesthesia period. Blood samples were taken from patients ۴۸ hours before surgery and ۲۴ hours after surgery. All samples froze at -۲۰°c immediately after collecting. Then samples transferred to the university laboratory with regards to keeping a cold chain for analysis. Paired T-test and Wilcoxon test were used for comparing vitamin B۱۲, homocysteine, and CBC parameters. Nitrous oxide resulted in marked vitamin B۱۲ and homocysteine level change. Mean concentration of vitamin B۱۲ reduced after exposure to N۲O. This difference was significant when analyzed using paired sample T-test (p=۰.۰۰۰۱). homocysteine concentration means decreased after exposure to N۲O and this difference was statistically significant (p=۰.۰۰۰۱). Nitrous oxide will lead to a decrease in vitamin B۱۲ and an increase in homocysteine and mean corpuscular volume in the early postoperative period.

کلیدواژه ها:

Analgesic ، Homocysteine ، Nitrous oxide ، Vitamin B۱۲