Study on Rooting and Changes of Some Secondary Metabolites of Dog Rose Grown in Lorestan Province

The dog rose medicinal shrub is one of the hardest rooting plants in Iran. This study was laid out in order to study on rooting and secondary metabolites of dog rose growes in different regions of Lorestan province. This study was performed in two experiments. In the first  experiments, the effect of different concentrstion of IBA was evaluated on rooting ability of dog rose. This study was performed by factorial design with two factors: ۱- cuttings at three levels (cuttings from regions including Abestan, Kakarza and Shul-Abad) ۲- Indole-۳-butyric acid (IBA) at ۴ levels (۰, ۲۰۰, ۱۰۰۰ and ۴۰۰۰ ppm) with three replications in March ۲۰۱۶. In the second  experiment, antioxidant ability and secondary metabolites produced by rooted cutting of dog rose cultivated in three region of lorestan province was studied. After rooting of doge rose hardwood cuttings were planted in two hot and cold regions of Lorestan province and secondary metabolites was measured after fruit harvesting. The results showed that the maximum rooting was recorded in ۴۰۰۰ppm IBA by ۶۱, ۵۹ and ۶۰.۵% for Abestan, Kakareza and Shul-Abad regions, respectively. In three regions, the minimum rooting percentage was recorded in control treatment by of ۱۱.۹, ۸ and ۱۱.۳% for them, respectively. Also the higher number of roots and leaves per cutting was recorded in ۴۰۰۰ppm IBA. Based on the results doge rose was not entrance in fruit set stage in warm Tajere-Sadat region. At Ab-sefid and Kuh-Kela regions that fruit set was performed, highest phenolic and flavonoid was founded in fruit skin by ۱۷.۱۲ and ۳.۲۳% for Ab-Sefid and ۱۶.۷۲ and ۲.۲۹% for Kuh-Kela regions respectively. Also lowest condensed tannins (۰.۶ and ۰.۶۵%) and highest DPPH activity (۳۶۲ and ۴۵۴ μg/mL) was founded in dog rose fruit and seed at Ab-Sefid and Kuh-Kela regions, respectively.

کلیدواژه ها:

antioxidant capacity ، condensed tannins ، flavonoid ، rooting