Predicting the negative regulation of ITM۲Cby miR-۲۱۸ in prostate cancer progression and metastasisby TCGA data analysis

Background: MicroRNAs (miRNAs) are small noncodingregulatory RNAs (۱۹–۲۵ nucleotides) that play a major role inregulation of gene expression. They are involved in controlling fundamental cellular processes that have been reported to beresponsible for human tumorigenesis. The characterization ofmiRNA profiles in human tumors is crucial in order to betterunderstand carcinogenesis, find new tumor markers, and discoverspecific targets for the development of novel therapies.The aim of this study is to find miRNAs involved in prostatecancer progression through comparing the profile of expressedmiRNA by localized high grade carcinoma and bone metastasis.miR-۲۱۸ was found to be significantly overexpressed byall localized high GS, pT۳ PC in compared to the metastaticcarcinoma.Materials and Methods: In this project, after the prediction ofmiRNA target gene by mirwalk database, we analyzed TCGARNA-seq data and predicted gene expression patterns.Result: The results of Mirwalk analysis show a strong interactionof miR-۲۱۸ with ITM۲C, and the results of prostate cancergenomics data analysis indicate a significant decrease in the expressionof ITM۲C in tumor samples compared to the normalsamples. The decrease in ITM۲C expression in metastatic samplesis also higher than non-metastatic samples.Conclusion: According to the binding site of miR-۲۱۸ on ITM۲CmRNA and the converse relationship between increasedmiR-۲۱۸ expression and decreased ITM۲C expression in tumorsamples, as well as decreased ITM۲C expression and increasedmiR-۲۱۸ expression in metastatic samples, regulation of ITM۲Cexpression by miR-۲۱۸ can be deducted.

کلیدواژه ها:

miR-۲۱۸ ، ITM۲C ، prostate cancer ، Metastasis ، TCGA