The effects of H۱۰۴۷R mutation of PIK-۳CA gene on the activity of PI۳K/Akt signaling pathway inbreast cancer cell line
- سال انتشار: 1402
- محل انتشار: اولین کنگره بین المللی ژنومیک سرطان
- کد COI اختصاصی: CGC01_121
- زبان مقاله: انگلیسی
- تعداد مشاهده: 119
نویسندگان
Department of Molecular Genetics, Faculty of Biological Sciences,Tarbiat Modares University, Tehran, Iran
Department of Biochemistry, Faculty of Biological Sciences, TarbiatModares University, Tehran, Iran
Department of Molecular Genetics, Faculty of Biological Sciences,Tarbiat Modares University, Tehran, Iran
چکیده
Background: PI۳Ks are a group of lipid kinases that phosphorylatethe ۳-hydroxyl group in the inositol ring of phosphatidyl-inositol molecules. Considering the very importantrole of the PI۳K/Akt signaling pathway in the cell, it is notsurprising that mutations in these kinases cause many diseases,cancer in particular. About ۴۰% of ER+/HER۲- breastcarcinomas have mutations in the PIK۳CA gene. One of theseclinically important mutations is H۱۰۴۷R, which occurs inexon ۲۰ of this gene.Methods and Results: First, the H۱۰۴۷R SNP was chosen to be produced in MCF۷ cell line by the utilization of CRISPR-Cas۹technology. Afterward, the subsequent tests were performed todeclare that producing isogenic mutants had been successful.Eventually, a sequencing analysis confirmed this claim. Successively,in order to analyze the effect of this mutation on theactivity of the PI۳K/Akt signaling pathway, western blot analysiswas accomplished.Conclusion: Identifying the effect of different mutations of thePIK۳CA gene involved in ER+/HER۲- breast cancer is a crucialstep to achieving an efficient pharmaceutical administration.With the isogenic mutants being available, it becomes possibleto conduct many intelligent and new Scientific studiesکلیدواژه ها
PIK۳CA, H۱۰۴۷R, CRISPR-Cas۹, Breast cancer,PI۳K/ Aktمقالات مرتبط جدید
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