Evaluation of dietary betaine on post-thawed semen quality in mature bulls during summer heat stress
محل انتشار: گفتمان پژوهش دامپزشکی، دوره: 13، شماره: 1
سال انتشار: 1401
نوع سند: مقاله ژورنالی
زبان: انگلیسی
مشاهده: 110
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شناسه ملی سند علمی:
JR_VRFAN-13-1_009
تاریخ نمایه سازی: 25 آبان 1402
چکیده مقاله:
Heat stress (HS) has caused relative hypoxia, oxidative stress and high level of homocysteine, which contributes significantly to fertility failures in bulls. The aim of present study was to evaluate the role of dietary betaine (BET) in improving dual purpose Simmental (Fleckvieh) post-thawed semen quality especially during the hottest summer days. A total number of ۱۶ mature bulls were randomly assigned to three equal groups including: ۱) Control condition (without betaine), ۲) BET۱: ۵۷.۰۰ mg of betaine kg-۱ per day and ۳) BET۲: ۱۱۴ mg of betaine kg-۱ per day, through daily intakes for ۹۰ days in summer. Plasma levels of homocysteine, seminal plasma antioxidants levels and sperm parameters such as DNA fragmentation, chromatin integrity, motility, viability, morphology and membrane integrity were evaluated. Under maximal HS, serum homocysteine concentrations were reached ۱۶.۶۷ ± ۰.۰۹ µmol L-۱. Dietary betaine supplementation influenced DNA fragmentation of sperm and was higher in the control group compared to BET۲ group. There were significant decreases in seminal plasma superoxide dismutase (SOD), glutathione peroxidase (GPx) activity and sperm viability and motility in bulls treated with betaine. The activity of GPx and SOD in the control group was increased up to ۰.۰۸ ± ۰.۰۰ U mg-۱ protein and ۰.۵۲ ± ۰.۰۱ U mg-۱ protein in seminal plasma. There were no significant differences between groups in the percentage of swollen spermatozoa, membrane integrity, sperm morphology, abnormal head morphology and percentage of spermatozoa stained with aniline blue. In conclusion, BET supplements improved semen parameters in sperm motility, sperm viability and influenced DNA fragmentation during HS with reduction in serum homocysteine concentrations.
کلیدواژه ها:
نویسندگان
Reza Ijab
Department of Theriogenology and Poultry Disease, Faculty of Veterinary Medicine, Urmia University, Urmia, Iran
Esmail Ayen
Department of Theriogenology and Poultry Disease, Faculty of Veterinary Medicine, Urmia University, Urmia, Iran
Amir Khaki
Department of Clinical Sciences, Faculty of Veterinary Medicine, Amol University of Special Modern Technologies, Amol, Iran
Ali Soleimanzadeh
Department of Theriogenology and Poultry Disease, Faculty of Veterinary Medicine, Urmia University, Urmia, Iran
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