Mutation and up-regulation of TP۵۳ in ovine pulmonary adenocarcinoma lung cells as a model of human lung cancer

Ovine pulmonary adenocarcinoma (OPA) is a model of human lung cancer‎ and fatal viral disease that causes neoplasia in sheep respiratory cells. ‎In the current study, ۹۸۶ lung samples was inspected in the slaughterhouse, and finally twenty OPA ‎ lung organs were clinically diagnosed and five healthy lung organs were assigned as the control sample. Three SSCP patterns were detected for the affected lungs animals in comparison with the healthy lungs. In addition, sequencing results indicated three different single point mutations in exon ۴ of TP۵۳ within infected lungs, whereas no mutations were observed in exon ۹ of this gene. Real-time PCR results showed up-regulation of the TP۵۳ gene in all the infected lung cells compared to healthy cells. There was significant correlation between the mutations in exon ۴ and OPAand can be used as a useful tool in determining the mechanism of lung cancer.

کلیدواژه ها:

Mutation ، Ovine pulmonary adenocarcinoma ، pathology ، TP۵۳ gene ، up-regulation