Avian gastric yeast (macrorhabdosis) in cockatiel, budgerigar and grey parrot: a focus on the clinical signs, molecular detection and phylogenetic evaluation
محل انتشار: گفتمان پژوهش دامپزشکی، دوره: 14، شماره: 5
سال انتشار: 1402
نوع سند: مقاله ژورنالی
زبان: انگلیسی
مشاهده: 68
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شناسه ملی سند علمی:
JR_VRFAN-14-5_006
تاریخ نمایه سازی: 25 آبان 1402
چکیده مقاله:
Macrorhabdus ornithogaster is a microorganism that causes nonspecific and general clinical symptoms and to this day, diagnosis and also treatment have been yet hard. The present study was conducted to survey the prevalence of macrorhabdosis and to characterize M. ornithogaster phylogenetically in Psittaciformes suspected of macrorhabdosis from January ۲۰۱۸ to May ۲۰۱۹ in Ahvaz, Iran. For this purpose, fecal samples were collected from Psittaciformes with signs of the disease. Wet mounts were prepared from fecal samples and examined carefully using a light microscope. Samples from parrots with gastrointestinal symptoms of the disease were chosen for molecular diagnosis of the organism and DNA was extracted from these samples. For detection of M. ornithogaster, primer sets (BIG۱, Sm۴) and (AGY۱, Sm۴) which target the ۱۸S rDNA gene were selected and Semi-nested polymerase chain reaction (Semi-nested PCR) was performed. The PCR method confirmed the presence of M. ornithogaster in ۱۴.۰۰% of the samples. Purified PCR products were sequenced for more accurate confirmation and according to the gene sequence all sequences were owned by M. ornithogaster. The results disclosed a ۹۶.۰۳% - ۱۰۰% identity when compared to other sequences of M. ornithogaster which had previously been deposited in the GenBank® from Germany and the USA. The results of this study proved the circulation of M. ornithogaster between cockatiel, budgerigar and grey parrot. The prevalence of macrorhabdosis was higher in cockatiel compared to budgerigar and grey parrot. As far as the authors know, this was the first record of macrorhabdosis in African grey parrots.
کلیدواژه ها:
نویسندگان
Forough Talazadeh
Department of Clinical Sciences, Faculty of Veterinary Medicine, Shahid Chamran University of Ahvaz, Ahvaz, Iran
Masoud Ghorbanpoor
Department of Pathobiology, Faculty of Veterinary Medicine, Shahid Chamran University of Ahvaz, Ahvaz, Iran
Yasaman Bahadori
Department of Clinical Sciences, Faculty of Veterinary Medicine, Shahid Chamran University of Ahvaz, Ahvaz, Iran
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