The profile of inflammatory factors in dairy calves with Cryptosporidium infection
محل انتشار: گفتمان پژوهش دامپزشکی، دوره: 13، شماره: 4
سال انتشار: 1401
نوع سند: مقاله ژورنالی
زبان: انگلیسی
مشاهده: 113
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شناسه ملی سند علمی:
JR_VRFAN-13-4_020
تاریخ نمایه سازی: 25 آبان 1402
چکیده مقاله:
The present study was conducted to investigate the detection and identification of Cryptosporidium species via molecular techniques and evaluate the serum concentrations of inflammatory factors in Cryptosporidium species. The fecal samples (n = ۲۵۶) were collected from pre-weaned (≤ ۲.۰۰ months) calves and the positive samples were identified utilizing Ziehl-Neelsen staining. Nested species-specific multiplex PCR (nssm-PCR) and restriction fragment length polymorphism (RFLP) were used to identify the species and sub-species. The serum concentrations of IL-۱β, IL-۶, IL-۱۲, TNF-α, and IFN-γ were also assessed. The results revealed that ۱۰.۵۴% of samples were positive. The results of Nested-PCR showed that ۹۲.۵۹% of the samples were positive for C. parvum while ۷.۴۱% were positive for C. andersoni. The results of RFLP confirmed ۹۲.۵۹% of the samples for C. parvum, ۳.۷۰% for C. muris / C. andersoni, and ۳.۷۰% for C. muris. The serum concentrations of IL-۱β, IL-۶, IL-۱۲, TNF-α, and IFN-γ were significantly higher in the infected calves compared to those in healthy calves. However, the serum concentration of IFN-γ was significantly higher in the calves infected with C. parvum while the serum concentrations of TNF-α and IL-۶ were significantly higher in those infected with C. andersoni. In conclusion, C. parvum was prevalent in the region and the calves demonstrated inflammatory responses to Cryptosporidium species.
کلیدواژه ها:
نویسندگان
Mostafa Meshkat
Department of Pathobiology, Faculty of Veterinary Medicine, Science and Research Branch, Islamic Azad University, Tehran, Iran
Bahar Shemshadi
Department of Pathobiology, Faculty of Veterinary Medicine, Science and Research Branch, Islamic Azad University, Tehran, Iran
Kumarss Amini
Department of Microbiology, Faculty of Basic Sciences, Saveh Branch, Islamic Azad University, Saveh, Iran
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