Effects of dietary vitamin E on mucosal maltase and alkaline phosphatase enzyme activities and on the amount of mucosal malonyldialdehyde in broiler chickens

The effects of dietary vitamin E levels on mucosal maltase and alkaline phosphatase (ALP) enzyme activities and on the amount of mucosal malonyldialdehyde (MDA) in broiler chickens were studied in the present study. One hundred and eighty of day old male broiler chicks (Ross ۳۰۸ strain) were randomly assigned into five groups, each with three replicates and ۱۲ chicks in each replicate. Chickens in group A were fed corn-soy- based diet, while those in groups B, C, D and E were fed the same diet with ۲۰, ۶۰, ۱۸۰, and ۵۴۰ mg kg-۱ vitamin E supplement (d-alpha tocopherol), respectively. Six birds were randomly chosen from each group, and were euthanized on days ۱۰, ۲۱, ۳۲, and ۴۲ of age. One segment of small intestine outset was homo-genized and mucosal ALP and maltase activity were measured. Moreover, mucosal lipid peroxidate amount was measured to reveal the impact of vitamin E on oxidative stress. Maltase activity was increased with the increase of vitamin E up to ۶۰ mg kg-۱ of diet while with further levels, it was decreased. Addition of ۶۰ mg kg-۱ of vitamin E to the diet significantly increased ALP enzyme activity (p ≤ ۰.۰۰۱). Addition of ۵۴۰ mg kg-۱ of vitamin E supplement to the diet led to the minimum amount of MDA at ۳۲ days of age. It may be concluded that supplementation of broiler's diet with ۶۰ mg kg-۱of vitamin E can increase mucosal maltase and ALP enzyme activity.

کلیدواژه ها:

Alkaline phosphatase ، Broiler chicken ، Malonyldialdehyde ، Maltase ، Vitamin E