Efficacy of mixture of emamectin benzoate with some insecticides on the mortality and esterase activity of fourth instar larvae of Tuta absoluta (Lepidotera: Gelechiidae)

The tomato leafminer, Tuta absoluta Meyrick (Lepidoptera: Gelechiidae) is one of the most damaging tomato pests in the world and in Iran. The toxicity of acetamiprid, eforia (thiamethoxam + lambda-cyhalothrin) and hexaflumuron alone and in mixture with emamectin benzoate was studied against ۴th-instars of Tuta absoluta (Meyrick) at ۲۵ ± ۲ °C, ۶۵ ± ۵% RH and ۱۶:۸ (L:D) h. Moreover, the mixtures of examined insecticides with emamectin benzoate at LC۱۵:LC۱۵ ratio were assessed on the general esterase enzyme activity and total protein content of ۴th-instars. The highest toxicity was found for emamectin benzoate after ۷۲ h (LC۵۰ = ۰.۴۸ mg a.i./l), followed by acetamiprid (LC۵۰ = ۴۶.۹۴ mg a.i./l), eforia (LC۵۰ = ۱۵۶.۲۴ mg a.i/l) and hexaflumuron (LC۵۰ = ۶۷۰.۳۲ mg a.i/l). Mixing emamectin benzoate with acetamiprid at the ratio of LC۵۰:LC۵۰ and LC۲۵:LC۲۵ resulted in synergistic impacts while mix of two other ratios of the same pesticides represented additive influences. The mixture of emamectin benzoate with either hexaflumuron or eforia at all ratios created antagonistic and additive effects, respectively. Mixing emamectin benzoate with either acetamiprid or eforia increased larval esterase activity, however, there was no significant difference between emamectin benzoate in mixture with hexaflumuron and using it alone. Mixing emamectin benzoate with the examined insecticides considerably decreased the larval total protein content. Based on the findings of this work, the mixtures of eforia and acetamiprid with emamectin benzoate represented greater negative effects against ۴th-instars compared to emamectin benzoate alone and the control.

کلیدواژه ها:

mixture effects ، emamectin benzoate ، acetamiprid ، eforia ، Tuta absoluta