Green Synthesis of Bismuth Oxide Nanoparticle by cell-free extract of Spirulina Platensis and evaluation of its performance on expression of apoptosis regulator genes in MCF-۷ breast cancer cell line

Background and Aim: Bismuth and its related compounds such as bismuth oxide nanoparticles (Bi۲O۳ NPs) are widely used in the medical field of science for drug delivery, cancer therapy, bio imaging, and fighting against pathogenic microorganisms. Most of the chemical compounds used in the synthesis of nanoparticles are toxic to human health and the environment, for this reason, recently considerable interest has been focused on the green synthesis of non-toxic and environmentally friendly nanoparticles (NPs). In the biosynthesis procedure, chemicals reducing agents are replaced by cell extract of organisms that contain bio-reductive compounds as stabilizing and capping agent for NPs. Methods: In this study, bismuth oxide nanoparticles (Bi۲O۳ NPs) were prepared using green synthesis by cyanobacterial cell extract of Spirulina platensis. The physico-chemical properties of the Bi۲O۳ NPs characterized by X-ray diffraction (XRD), energy dispersive X‒ray (EDX) analysis, Fourier-transform infrared (FT-IR) spectroscopy, scanning and transmission electron microscopy (SEM). The apoptotic properties of biosynthesized nanoparticles on MCF-۷ and MCF-۱۰a cell lines were determined using the effect of the Bi۲O۳ NPs on the expression of BAX and BCL۲ genes by real-time polymerase chain reaction. Results: The size, shape, and morphology of Bi۲O۳ NPs are suitable for penetrating into the cancer cells and affecting their vital activities. In the MCF-۷ cell line, the ratio of BAX/BCL۲ expression was significantly higher than that of in MCF-۱۰a cell line treated with the Bi۲O۳ NPs. Conclusion: The results revealed the stimulation of apoptosis in breast cancer cell line by green synthesized Bi۲O۳ NPs through an increase in apoptosis regulator genes expression.

کلیدواژه ها:

BAX ، BCL۲ ، Bi۲O۳ NPs ، Breast cancer ، Green synthesis ، Spirulina Platensis