Study on the resistance to chlorpyrifos in Microcerotermes diversus (Isoptera: Termitidae)

Microcerotermes diversus Silvestri, is an important termite in Khuzestan province with a broad range of foraging. In recent years, control of underground termites has been more based on the application of chemicals which has increased pest insecticide resistance. In this study, the resistance of different populations of termites to chlorpyrifos was investigated using estimated LC۵۰ and measuring the activity of two acetylcholinesterase (AChE) and glutathione-s-transferase enzymes. Four populations of M. diversus with different histories of organophosphorus insecticide spraying were studied. Populations A (Am Altamir۱), B (Am Altamir۲), and C (Mollasani) were collected from date palm groves with ۳۰ and ۱۰-year histories of organophosphorus insecticide spraying, as well as without spraying history, respectively. Population D (Ramin University) was collected from orange trees, three weeks after spraying with chlorpyrifos. The bioassay results showed that the highest and lowest LC۵۰ values were observed in populations A and C, respectively. Moreover, the activity of AChE using acetylthiocholine iodide, propionylthiocholine iodide, and butyrylthiocholine iodide substrates was higher in the populations with chlorpyrifos spraying history, with the highest activity in population A. It was also demonstrated that the AChE activity with acetylthiocholine iodide substrate was higher than the activity of this enzyme with butyrylthiocholine iodide and the propionyl choline substrates in the populations with ۳۰ and ۱۰-year history of spraying. The Michaelis constant (Km) and maximum velocity (Vmax) values of AChE as two important kinetic factors, indicated the highest affinity of this enzyme to the substrate in population A.

کلیدواژه ها:

Acetylcholinesterase ، Glutathione-S-transferase ، Insecticide resistance ، Organophosphorus insecticide ، Termite