Analysis of VTRNA ۱-۲ expression in patients with colorectal cancer

VaultRNAs are a part of noncoding RNAs (ncRNAs) involved in transcription, translation, proliferation, and regulation of important cellular pathways such as cell death (apoptosis and autophagy), cell differentiation, and cell-to-cell communication. vtRNA۱-۲ gene expression is suppressed in tumors compared to normal tissues due to the high level of promoter methylation(۱). It has been suggested that vtRNA۱-۲ should be a tumor suppressor gene. The purpose of this study was to analyze the expression pattern of vtRNA۱-۲ in patients with colorectal cancer (CRC) compared with healthy individuals using Real time-PCR. Extracted RNA will be quantified using nano-drops at wavelengths of ۲۶۰ and ۲۸۰ nm. The total RNA was extracted and reverse-transcribed into cDNA before qRT‐PCR. The vtRNA۱-۲ cycle threshold (Ct) values were normalized to glyceraldehyde ۳-phosphate dehydrogenase (GAPDH) as housekeeping gene control. Quantitative measurements were performed in triplicate and relative expression was measured using the comparative Ct method (۲−ΔΔCt). Statistical data were analyzed using GraphPad Prism (version ۹.۰.۲) and were presented as mean ± standard deviation. The differences were considered significant when the p-value was less than ۰.۰۵. Total RNA was successfully extracted from all samples. In addition, ۵.۸S, ۱۸S, and ۲۸S ribosomal RNA bands were detected after electrophoresis of total RNA on ۱.۵% agarose gel, indicating that the total RNA obtained was suitable for RNA amplification. The qRT-PCR analysis revealed that the relative level of vtRNA۱-۲ expression was significantly higher in cases compared to normal (p<۰.۰۰۵). In addition, there was not any association between the expression level of the vtRNA۱-۲ gene with the stages of CRC. Based on the result of this study, change in vtRNA۱-۲ expression may be important in the development or progression of CRC. However, further work is needed to clarify the underlying molecular mechanism(۲).

کلیدواژه ها:

VaultRNAs ، VTRNA۱-۲ ، Gene expression ، Colorectal cancer