Synthesis of folic acid-conjugated tragacanth gum polymeric micelles for piperine delivery

Piperine has attracted the attention of medicinal chemists and health professionals due to its various physiology function (۱). However, despite all the promising properties, the solubility of piperine is very poor in aqueous solution and digestion and absorption of it is difficult, leading to its poor bioavailability and limited therapeutic effect for disease treatment (۲). In this work, piperine was isolated from black pepper and then purified and identified by FT-IR and ۱HNMR spectral data and its melting point (۱۲۷-۱۳۰ °C) and comparison with data recorded in the literature (۳). In next step, folic acid-conjugated tragacanth gum polymeric micelles were successfully prepared. This polymeric micelles is suitable for encapsulation of piperine. The polymeric micelles were physicochemically characterized by FT-IR, XRD and AFM. The functionalized polymeric micelles were obtained in spherical shape with diameter about of ۲۰۰ nm. In vitro release analyses, antioxidant activity, hemolysis assay and intrinsic cytotoxicity of MNPs were determined. The results showed that the prepared MNPs released higher amount of piperine in acidic medium compared to neutral medium. Also, according to hemolysis activity, prepared polymeric micelles as delivery system is hemocompatible with negligible hemolytic effects. This study shows that folic acid-conjugated tragacanth gum polymeric micelles as a carrier develops the biological activities of piperine and can be regarded in further bioapplications

کلیدواژه ها:

Folic acid ، Piperine ، Tragacanth Gum ، Micelles ، Polymer ، Drug delivery