۶Genetic association of HLA-G ۳'UTR polymorphism with risk of gastriccancer

HLA-G molecules exert inhibitory effects on both innate and adaptative immune responses, such as inhibition of natural killer (NK) cells, as well modulation of B and T cell function. Their interaction with immunoglobulin-like receptors, such as receptor Ig-like transcripts ۲ and ۴ (ILT۲, ILT۴) and killer cell immunoglobulin-like receptor (KIR) KIR۲DL۴ expressed by peripheral NK cells, B- and T lymphocytes and monocytes. We selected a ۱۴bp polymorphism rs۳۷۱۱۹۴۶۲۹ for genotype frequency analysis and assessed the frequency of HLA-G gene polymorphism in ۲۰ gastric cancer patients and ۴۰ randomly selected control subjects.All clinical data were collected at the time of enrollment. The Whole blood samples were collected from all control subjects and patients. The buffy coat was separated and kept frozen at -۸۰°C. Genomic DNAs were extracted from peripheral blood leukocytes using proteinase K and phenol/chloroform. PCR was performed using a set of primers following the RFLP method. The strength of the association between HLA-G gene polymorphisms and the risk of gastric was assessed by odds ratios (ORs) with ۹۵% confidence intervals (CIs) according to alleles and genotypes in case of group and observation. The frequencies of homozygote genotypes for the presence of (+۱۴bp/+۱۴bp)۱۴bp, heterozygote genotypes for (-۱۴bp/+۱۴bp), and homozygote genotypes with deletion of (-۱۴bp/-۱۴bp)۱۴bp segments in the healthy individuals were ۲۱.۵۷%, ۴۱.۱۸% and ۳۷.۲۵%, and in the patients were ۱۷.۵%, ۴۲.۵% and ۴۰% respectively. The most frequent genotype in the healthy individuals and heterozygote patients was (-۱۴bp/+۱۴bp). We found no statistically significant differences in the genotype distributions between the cases and controls.۵۹

کلیدواژه ها:

gastric cancer ، HLA-G ، polymorphism ، rs۳۷۱۱۹۴۶۲۹