Advances in CRISPER/Cas۹ technology

Genome editing is an important biotechnological tool that enables genetic engineering of many living organismsby inserting, deleting, or replacing DNA at a precise location in the genome. The most commonly used technologies for this purpose include transcription activator-like effector nucleases (TALENs), endonucleases or meganucleases homing, zinc finger nucleases (ZFNs), and clustered regularly interspaced short palindromic repeats (CRISPR) associated protein ۹. Among these tools, CRISPR/Cas۹ is preferred more. The CRISPR/Cas۹ system can specifically identify any target and activate mutation correction or expression regulation through the NHEJ or HDR pathway, providing a powerful platform for revolutionary treatments of various diseases. CRISPR applications have not only grown rapidly but also transformed biological sciences. Currently, this technology is being used in various fields such as agriculture, food biotechnology, medicine, cancer, and targeted genome therapies. In particular, these tools have provided an extraordinary new avenue for gene therapy and cell therapy in particular.

کلیدواژه ها:

Genome Editing ، Molecular Markers ، CRISPR/Cas۹