Effect of the complex of β-glucan and Zataria multiflora on oxidative burst of neutrophils in BALB/c mice

Natural products such as β-glucan and herbal essences have been found to enhance immune functions. The aims of this study were to purify β-glucan from the cell wall of Saccharomyces cerevisiae and to investigate its conjugate effect with Zataria multiflora essence on oxidative burst in BALB/c mice. After preparing the cell walls from the yeast, β-glucan was extracted from wall by alkaline-acid treatment (glucan-S۱). This preparation contained protein and mannan, which were removed by DEAE sephacel chromatography (glucan-S۲) and con-A sepharose chromatography (glucan-S۳), respectively. Glucan-S۳, Z. multiflora essence, and their conjugate were injected into BALB/c mice intraperitoneally. Blood was collected at days ۴ and ۷ after injection and oxidative burst was assayed by chemiluminescence method. The results showed that intraperitoneally administration of glucan-S۳, Z. multiflora essence and their conjugate significantly increased oxidative burst of neutrophils both at days ۴ and ۷ when compared to control group (P<۰.۰۵). These findings suggest that the complex of β-glucan and Z. multiflora essence can be used as adjuvant agents to stimulate immune functions in immunocompromised subjects.

کلیدواژه ها:

Saccharomyces cerevisiae ، β-glucan ، Zataria multiflora essence ، oxidative burst.