Introduction: A chronic wound can be described as a wound that cannot heal in the expected time frame (of less than ۳ months). The ۵-year mortality rate of diabetic foot ulcers is ۳۰.۵%, which is comparable to the mortality rate linked with cancer (۳۱%) Management of chronic
diabetic wounds remains problematic due to the lack of diagnostic tools allowing the distinction between colonization and infection of the wounds. ۵۰% of patients that develop clinically overt infection require amputation. Method: In the forthcoming systematic study, the required data were collected using keywords and citing valid databases such as: Scopus PubMed, Google Scholar and ProQuest. The statistical population of the study includes all studies conducted up to ۲۰۲۲ in the field of The role of the microbiome in nonhealing diabetic wounds. After reviewing the relevant findings and evaluating the data quality, a total of ۲۵ articles were analyzed.Results: Biofilm increases virulence, reduces treatment success, offers a physical barrier to biological and antimicrobial substances, facilitating microorganism attachment to surfaces or to each other. In biofilm, anti-proliferative signals derived from pathogens aborts fibroblasts and keratinocyte functions. Thus, the consequence is failure in granulation tissue formation and the re-epithelialization trajectory. Among the common species, Staphylococcus aureus is the most commonly observed species, followed by The negative. More evidence suggests that. Are common in wounds. Of beneficial microbiota is Staphylococcal SPP. S. biofilm, is one of them. Staphylococcus fibroblasts produces Thus which was shown to be effective in decreasing the nasal carriage of S. aureus. P. granulation and S. aureus secrete a plethora of proteases resulting in collagen, fibrinogen and elastin degradation, inhibition of PMNs. Conclusion: The microbiome is a therapeutic target that can be modified; a fundamental understanding of its components will reveal novel targets for managing and treating diabetic wounds. With its central role for microbiota, the gut-skin axis represents an exciting field of research with a wide range of therapeutic applications, including diabetes wound healing