Effects of Hydrogen Peroxide Oxidative Stress on the Pattern of Pro-apoptotic and Anti-apoptotic Genes Expression During PC۱۲ Cells Differentiation

Background and Aims:In neurodegenerative disorders,oxidative stress mediated by reactive oxygen species is strongly associated with increased neuronal damages which can lead to apoptosis. Pro-apoptotic and anti-apoptotic gene expressions are changed during the cell differentiation that affect cell viability and differentiation. Therefore, this study was conducted to determine the effects of hydrogen peroxide-induced oxidative stress on the apoptotic cell death in the differentiated rat pheochromocytoma (PC۱۲) cells. Materials and Methods:Semi-differentiated PC۱۲ cells were treated with ۴۰۰ µM hydrogen peroxide (H۲O۲). Characteristic morphological changes as apoptotic index were evaluated by DAPI staining. MTT assay were applied in order to evaluate the cells survival as well as cell activity.Pro-apoptotic and anti-apoptotic gene expressions were estimated by real time-PCR. Results:The obtained data indicated that PC۱۲ cell survival rate decreased H۲O۲ treated condition during the differentiation. Moreover, H۲O۲ was proved to increase apoptotic genes expressions including caspase-۶ as well as PIN۱ and to decrease anti-apoptotic genes including SIRT۱ as well as SIRT۷. Conclusions: The findings of the present study revealed that H۲O۲-induced oxidative stress can retard the differentiation of PC۱۲ cell in the form of neural-like cells through the apoptotic gene expression. On the other hand, although the PIN۱ acts as an apoptotic gene, this study illustrated that this gene expression can get increased during the differentiation under oxidative stress conditions

کلیدواژه ها:

Apoptosis ، Caspase-۶ ، PC۱۲ ، PIN۱ ، SIRT۱ ، SIRT۷