Iron Chelating Activity of Nepeta Crispa Willd., an Endemic Plant in the West of Iran
محل انتشار: مجله بیوشیمی پزشکی، دوره: 10، شماره: 1
سال انتشار: 1401
نوع سند: مقاله ژورنالی
زبان: انگلیسی
مشاهده: 158
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شناسه ملی سند علمی:
JR_MEBIO-10-1_009
تاریخ نمایه سازی: 28 تیر 1402
چکیده مقاله:
Background: Nepeta crispa Willd., a member of Lamiaceae family, is an annual plant native to western
Iran, especially Hamedan, with many traditional uses. The plant effects have not been investigated yet. Iron
chelating activity is a suitable test for measuring antioxidant activity.
Objectives: The present study aimed to evaluate Iron-chelating activity of extract, fractions, and essential
oil of the N. crispa, in vitro.
Methods: The methanolic extract of N. crispa was prepared adopting maceration method. Then, the total
methanolic extract was fractionated using hexane, chloroform, ethyl acetate, and water. The essential oil
was isolated by hydrodistillation using a Clevenger-type apparatus. Chelating activity of total extract, each
fraction and essential oil of N. crispa on Fe۲+ ions was determined using Iron-chelating assay. EDTA was
employed as positive control.
Results: EDTA as positive control had the best activity with IC۵۰ = ۰.۰۲ ± ۰.۰۰۱ mg/mL, and ascorbic acid
came second in this regard (IC۵۰ = ۰.۳۸۶ ± ۰.۰۲۱ mg/mL). The hexane fraction was the most active fraction
among the different fractions of N. crispa (IC۵۰ = ۰.۴۳۵ ± ۰.۰۳۲ mg/mL). Chelating activity of hexane
fraction was followed by aqueous, ethyl acetate, chloroform fractions, and methanolic extract with ICs۵۰
of ۱.۴۳۳ ± ۰.۰۹۸, ۲.۱۵۸ ± ۰.۰۷۴ mg/mL, ۳.۶۲۴ ± ۰.۱۱۲, and ۳.۰۵۱ ± ۰.۱۷۴, respectively. The essential oil
showed extremely poor activity in the tested concentrations.
Conclusion: It was concluded that the n-hexane fraction had promising Iron chelating activity, and was
likely capable of reducing Fe۲+ ions concentration and preventing oxidative damage.
کلیدواژه ها:
نویسندگان
Parisa Ranjbaran
Medicinal Plants and Natural Products Research Center, Hamadan University of Medical Sciences, Hamadan, Iran- Department of Pharmacognosy, School of Pharmacy, Hamadan University of Medical Sciences, Hamadan, Iran
Shirin Moradkhani
Medicinal Plants and Natural Products Research Center, Hamadan University of Medical Sciences, Hamadan, Iran- Department of Pharmacognosy, School of Pharmacy, Hamadan University of Medical Sciences, Hamadan, Iran