The Effect of Low-Level Laser Therapy on Differentiation Ability of Human Adipocyte-Derived Stem Cells to Keratinocytes
سال انتشار: 1402
نوع سند: مقاله ژورنالی
زبان: انگلیسی
مشاهده: 105
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شناسه ملی سند علمی:
JR_IJABBR-11-2_004
تاریخ نمایه سازی: 28 خرداد 1402
چکیده مقاله:
Background: Low-level laser therapy (LLLT), is a clinically well-established tool for wound healing. In vitro studies have shown that LLLT has abiostimulatory effect on mesenchymal lineages and non-mesenchymal lineages of Adipose-derived stem cells (ADSCs) differentiation. The aim of this study was to evaluate the effects of LLLT at different energy intensities on differentiation of (ADSCs) to keratinocyte.Methods: Adipose tissue were successfully obtained from ۴۵-year-old woman by Lipoaspirates and adipose-derived mesenchymal stem cells (ADSCs) were isolated. ADSCs were divided into a control group and three low-level laser treated groups irradiated with ۰.۵ J/cm۲, ۱ J/cm۲ and ۲ J/cm۲ laser doses using He-Ne laser with ۶۳۲nm wave length in ۵ times. All groups cultured with keratinocytes media for ۱۰-days. Cell proliferation was eveluated using ۳-(۴, ۵-dimethylthiazol-۲yl)-۲, ۵-diphenyltetrazolium bromide (MTT) assay. ADSCs developed a polygonal cobblestone shape characteristic of human keratinocytes. Specific keratinocyte marker ۵ (KRT۵) were analyzed by real-time PCR. Data were analyzed using one-way AVOVA and Tukey-Kramer hoc test by SPSS software version ۱۶.Results: Results of this study demonstrated LLLT could significantly affect proliferation of ADSCs (P<۰.۰۵) and promote ADSCs differentiation to keratinocytes by increasing expression of KRT۵ at of ۱ J/cm۲ and ۲ J/cm۲ laser doses (P<۰.۰۱). LLLT at density of ۰.۵ J/cm۲ could not promote ADSCs differentiation significantly.Conclusion: Considering these findings, LLLT could improve current in vitro methods of differentiating ADSCs to keratinocyte prior to transplantation. Thereby LLLT can use clinically as promoting skin wound healing.
کلیدواژه ها:
Adipose-derived stem cells (ADSCs) ، Low-level laser therapy (LLLT) ، Differentiation ، Keratinocyte ، KRT۵
نویسندگان
Zohreh Bahadorloo
Department of Biology, Faculty of Sciences, Central Tehran Branch, Islamic Azad University, Tehran, Iran
Zeinab Piravar
Department of Biology, Faculty of Sciences, Central Tehran Branch, Islamic Azad University, Tehran, Iran
Jamshid Sabaghzadeh
Department of Physics, Faculty of Sciences, Central Tehran Branch, Islamic Azad University, Tehran, Iran
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