Expression of GABAAα۱, GABAB۱, and mGluR۲ receptors in the lateral geniculate body of male neonates born to diabetic rats

Objective(s): Diabetes during gestation is one of the most common pregnancy complications and has adverse effects on offspring, including a negative impact on the offspring’s central nervous system (CNS). Diabetes is a metabolic disease associated with visual impairment. Due to the importance of the lateral geniculate body (LGB) in the visual pathway, the present study examined the effect of maternal diabetes on the expression of gamma-aminobutyric acid (GABAAα۱ and GABAB۱) and metabotropic Glutamate (mGlu۲) receptors in the LGB of male neonates of diabetic rats.Materials and Methods: Diabetes was induced in female adult rats by a single intraperitoneal dose of streptozotocin (STZ) ۶۵ (mg/kg). In the Insulin-treated diabetic rats, diabetes was controlled by subcutaneous NPH-insulin injection daily. After mating and delivery, male offspring were killed by carbon dioxide gas inhalation at P۰, P۷, and P۱۴ (postnatal days ۰, ۷, and ۱۴). The expression of GABAAα۱, GABAB۱, and mGluR۲ in the LGB of male neonates was determined using the immunohistochemistry (IHC) method.Results: The expression of GABAAα۱ and GABAB۱ was significantly reduced, whereas the expression of mGluR۲ was markedly increased in the diabetic group compared with the control and insulin-treated groups at P۰, P۷, and P۱۴.Conclusion: The results of the present study showed that induction of diabetes altered the expression of GABAAα۱, GABAB۱, and mGluR۲ in the LGB of male neonates born to diabetic rats at P۰, P۷, and P۱۴. Moreover, insulin treatment could reverse these effects of diabetes.

کلیدواژه ها:

Diabetes Mellitus ، GABA ، Geniculate bodies ، Glutamate metabotropic- receptors ، Metabotropic glutamate