Effect of resveratrol on hippocampal and cortical oxidative stress markers in LPS-induced neuroinflammation rats

Introduction and Objective: There is considerable evidence that neuroinflammation (NI) and stress oxidative may be involved in the pathophysiology of several neurodegenerative diseases. From these standpoints, some therapeutic strategies are designed to halt or at least slow down the progression of neurodegenerative diseases by alleviating oxidative stress and NI. Therefore, the present study aimed to investigate the impacts of resveratrol on hippocampal and cortical inflammation as well as oxidative stress markers in lipopolysaccharide (LPS)-induced NI in rat brains.Methods: The NI was induced in adult male Wistar rats (n=۱۸) by intracerebroventricular (ICV) injection of LPS (۵۰ μg/۲۰μl; ۱۰ μl into each ventricle). Meanwhile, the sham group(n=۶) only received ICV injection of vehicle (artificial cerebrospinal fluid). Resveratrol at doses of ۵mg/kg and ۳۰mg/kg were intraperitoneally administrated to LPS-induced NI rats (n=۶, each dose) ۳۰ min before the LPS injection and continued once per day up to ۴۸ h. Another LPS-induced NI group (n=۶) was only treated with normal saline containing ۱% ethanol and considered as a model group. On day ۳, animals were sacrificed and their prefrontal cortex and hippocampi were dissected. Then, the tissue concentrations of TNF-α, malondialdehyde (MDA), superoxide dismutase (SOD), and nitric oxide (NOx) were determined.Results: Resveratrol at the high dose (۳۰mg/kg) prevented both cortical and hippocampal MDA elevation and increased SOD activity but did not affect NOx contents. Resveratrol at both doses could significantly ameliorate TNF-α levels in both cortex and hippocampus of LPS-induced NI rats. Resveratrol only at the high dose (۳۰mg/kg) could normalize hippocampal and cortical levels of MDA, SOD and TNF-α.Conclusion: In conclusion, the results of the present study showed that systemic administration of resveratrol in a dose-dependent manner could effectively ameliorate oxidative stress and NI induced by LPS in rat brains.

کلیدواژه ها:

Innate Inflammatory Response ، Neurodegenerative diseases ، Oxidative stress ، Resveratrol