Ultrasensitive electrochemical biosensor for detection of serum HER۲ using nanometal as a signal amplification modifier

The main breast cancer-related protein biomarkers for non-invasive clinical tests are the Human Epidermal growth factor Receptor ۲ (HER۲ or ErbB۲), Cancer Antigen ۱۵-۳ (CA ۱۵-۳), and Carcinoembryonic Antigen (CEA) [۱] HER۲ stimulates the growth of breast cancer cells as a result the accurate detection of Human epidermal growth factor receptor-۲ (HER۲) as an important breast cancer biomarker can be essential for the early selection of therapeutic approaches [۲].Herein, we present a fast and simple method for the detection of serum HER۲. In this regard, a new electrochemical material has been developed, which is made up of composite of Ag and Au nanometals and Ni foamAmong different noble MNPs, silver and gold nanoparticles are subjected to new engineering technologies with extraordinarily novel resultant morphologies and characteristics. These NPs have several benefits including simplicity of the preparation process, cost-effective manufacturing method, facilitating more efficient electron transfer (eT), and harboring more active sites on their surface [۳]. These advantages make it a suitable choice, and it seems that utilizing they along with Ni foam as a specific platform can be a good choice in designing electrochemical sensors and aptasensors.The Ni foams exhibit excellent electrochemical stability in electrolytes and are compatible with various electrochemically active materials as potential structured. also the preparation approach of the Ni foams are facile, cost-effective, and can be used for large-scale production.In the current study, a novel and highly selective aptasensor was developed on an electrode surface for the ultrasensitive detection of HER۲ through an strategy of functionalized GCE with the Ni foam and (Ag and AuNPs) as an efficient nanocomposite. In the proposed sensing strategy, the aptamer was covalently attached on the Nano metals, which was in turn attached on the surface of a glassy carbon electrode (GCE) modified. By combining the advantages of each nanocomposites component like stability of Ni foam, excellent conductivity of AgNPs nanometal,the synthesized nanocomposite exhibited very well electrocatalytic activity in the electrode surface. The electrochemical measurements were investigated using cyclic voltammetry (CV).Furthermore, the electrode response changes at each stage after the modification with the nanomaterials, aptamer and HER۲ was efficiently investigated using EIS technique. Different concentrations of CEA were measured by aptasensor under optimized experimental conditions , and their current responses were recorded by the DPV method. the designed aptasensor showed a wide linear response range of ۱۰-۱۲-۱۰-۶ μg.mL−۱, the low detection limit of ۱۰ pg.mL−۱, and good selectivity and excellent detection of CEA in serum samples.Consequently, biosensor based on synthesized nanocomposite represented a collection of very favorable sensing performances, including desirable operating potential, low detection limit, high stability, sensitivity and reproducibility.

کلیدواژه ها:

Aptasensor ، HER۲ ، Breast cancer ، Ni Foam ، Ag and Au nanoparticles