Synthesis of Novel Fluorescent Imprinted Nanoparticles as Nanobiosensors

Biosensors have been used extensively in diagnostics, environmental monitoring and food processes. Because of some problems associated with biological molecules as receptors in biosensors, Molecularly imprinted polymers (MIPs) have been introduced as adequate alternative for them [1, 2].The interest in fluorescence biosensors is continually growing, as they provide a sensitive and selective method to recognize and evaluate the concentration of different substrates. On the other hand, for true non-invasive intracellular analysis, the sensor must be about 100 times smaller than the analyzed cell. Nanoparticles with embedded fluorescent dyes have potential as nanosensors for the detection of metabolite concentrations in living cells. The employment of a single fluorescent nanoparticle as an optochemical sensor was first demonstrated by Sasaki et al. in 1996 [3].To the best of our knowledge, there is no report on preparation of fluorescent imprinted nanoparticles. This paper describes our initial results of fluorescent glucose imprinted nanoparticles as a nanobiosensor for determination of glucose, which can be considered as new sensitive tool for diagnostics purpose