The immunophenotype of bone marrow lymphocytes in children with immunethrombocytopenic purpura

Purpose: Primary immune thrombocytopenic purpura (ITP), caused by immune systemdysfunction, is recognized as the leading cause of thrombocytopenia in pediatric population.Nonetheless, inadequate studies have been performed on bone marrow immunophenotyping ofchildren with ITP. In this study, we aimed to investigate the immunophenotype of bone marrowlymphocytes in these children.Patients and methods: Between ۲۰۰۸ and ۲۰۱۲, ۳۵ children with ITP and ۲۶ age and sex matchedhealthy controls were recruited. All participants underwent bone marrow aspiration. AppropriateB-cell, T-cell, and myeloid lineage monoclonal antibodies were employed to determine theimmunophenotype of these patients.Results: CD۱۰, CD۱۹, and CD۲۰, all indicative of premature B-cell markers, were significantlygreater in children with ITP. CD۲۲, mainly expressed on mature B cells was slightly, but notsignificantly reduced in the patients' group (P = .۴۲). On the other hand, T cell markers includingCD۲, CD۳, CD۵, and CD۷ were underexpressed. CD۳۳, a specific marker for myeloid lineage,was underexpressed in the patients' group (۵.۶ ± ۴.۷ vs. ۱۲.۹ ± ۷.۳, P < .۰۰۱). Noteworthy, theimmunophenotype did not significantly differ between acute and persistent cases.Conclusion: Overall, a phenotype characterized by increased pre-B-cell markers along withdecreased T cell immunophenotypic markers was observed in bone marrow lymphocytes ofchildren with ITP in the present study. Further larger scale studies are recommended to confirmour findings, as precise mapping of the immunophenotype of lymphocytes in these patients wouldpave the road to improved diagnosis and treatment.

کلیدواژه ها:

bone marrow lymphocytes ، cellular immunology ، childhood ، immunethrombocytopenic purpura ، immunophenotype.