Analysis of the Effect of Chimeric Chitinase Expressed by Synthetic Promoter in T۲ Generation of Transgenic Canola

سال انتشار: 1402
نوع سند: مقاله ژورنالی
زبان: انگلیسی
مشاهده: 225

فایل این مقاله در 11 صفحه با فرمت PDF قابل دریافت می باشد

استخراج به نرم افزارهای پژوهشی:

لینک ثابت به این مقاله:

شناسه ملی سند علمی:

JR_SGR-9-1_006

تاریخ نمایه سازی: 9 اسفند 1401

چکیده مقاله:

The chitinase enzymes are known to play an important role in the plant defense system against phytopathogenic fungi. The effect of chimeric chitinase, which is chitinase-۴۲ with a chitin-binding domain (ChBD), was previously analyzed in the T۰ generation of the transgenic canola. In this research, three homozygous lines (pGFC۳, pGFC۱۳, and pGFC۲۶) containing a single copy of the transgene (chimeric chitinases) on the two homologous chromosomes were selected in the T۲ generation using a kanamycin-resistant marker (NPTII gene). The selected homozygous plants in T۲ generation were induced by chitin as an elicitor in the greenhouse. The results of the semi-quantitative RT-PCR, chitinase enzyme activity, and growth inhibition of phytopathogenic fungi demonstrated that the synthetic inducible promoter of transgenic plants was induced by chitin. The results of chitinase activity of extracted protein from all transgenic lines containing inducible promoters showed a ۳.۲-۵.۸-fold increase in chitinase activity compared to non-induced plants. The antifungal activity of the inducibly expressed chitinase was examined on Sclerotinia sclerotiorum and Rhizoctonia solani. The results showed that fungal growth inhibition increased via elicitor treatment of the inducible promoter, ۸۲% for S. sclerotiorum and ۶۲% for R. solani, respectively. The result of light microscopic observation demonstrated morphological changes in hyphae and that the expressed enzyme can lyse the mycelial cell walls of R. solani. Moreover, resistance to S. sclerotiorum in the intact leaves of transgenic plants (T۲) was confirmed using bioassay analysis. Based on these results, it seems that the synthetic inducible promoter containing F cis-acting element driving chimeric chitinase is suitable for increasing the resistance of the canola transgenic plant when attacked by phytopathogenic fungi.

نویسندگان

Mehdi Moradyar

Department of Plant Biotechnology, National Institute of Genetic Engineering and Biotechnology, Tehran, Iran

Mohammadreza Zamani

Department of Plant Biotechnology, National Institute of Genetic Engineering and Biotechnology, Tehran, Iran

Mostafa Motallebi

Department of Plant Biotechnology, National Institute of Genetic Engineering and Biotechnology, Tehran, Iran

Esmat Jourabchi

Department of Plant Biotechnology, National Institute of Genetic Engineering and Biotechnology, Tehran, Iran

مراجع و منابع این مقاله:

لیست زیر مراجع و منابع استفاده شده در این مقاله را نمایش می دهد. این مراجع به صورت کاملا ماشینی و بر اساس هوش مصنوعی استخراج شده اند و لذا ممکن است دارای اشکالاتی باشند که به مرور زمان دقت استخراج این محتوا افزایش می یابد. مراجعی که مقالات مربوط به آنها در سیویلیکا نمایه شده و پیدا شده اند، به خود مقاله لینک شده اند :
  • Aghazadeh R, Zamani M, Motallebi M, Moradyar M, Moghadassi Jahromi ...
  • Broekaert WF, Delauré SL, De Bolle MFC, Cammue BPA. ۲۰۰۶. ...
  • Cazzonelli CI, Velten J. ۲۰۰۸. In vivo characterization of plant ...
  • Chernin L, Ismailov Z, Haran S, Chet I. ۱۹۹۵. Chitinolytic ...
  • Chernin LS, De la Fuente L, Sobolev V, Haran S, ...
  • Chet I, Inbar J. ۱۹۹۴. Biological control of fungal pathogens. ...
  • Gunaratna K.R, Balasubramanian R. ۱۹۹۴. Partial purification and properties of ...
  • Gurr SJ, Rushton PJ. ۲۰۰۵. Engineering plants with increased disease ...
  • Hammond-Kosack KE, Parker JE. ۲۰۰۳. Deciphering plant–pathogen communication: fresh perspectives ...
  • Harighi MJ, Motallebi M, Zamani MR. ۲۰۰۶. Antifungal activity of ...
  • Heise A, Lippok B, Kirsch C, Hahlbrock K. ۲۰۰۲. Two ...
  • Joosten MHAJ, Verbakel HM, Nettekoven ME, van Leeuwen J, van ...
  • Khademi M, Varasteh-Shams M, Nazarian-Firouzabadi F, Ismaili A. ۲۰۲۰. New ...
  • Kobayash DY, Reedy RM, Bick J, Oudemans P.V. ۲۰۰۲. Characterization ...
  • Kunz C, Sellam O, Bertheau Y. ۱۹۹۲. Purification and characterization ...
  • Limón MC, Chacón MR, Mejías R, Delgado-Jarana J, Rincón AM, ...
  • Lorito M, Farkas V, Rebuffat S, Bodo B, Kubicek CP. ...
  • Lorito M, Woo SL, Fernandez IG, Colucci G, Harman GE, ...
  • Matroodi S, Motallebi M, Zamani M, Moradyar M. ۲۰۱۳. Designing ...
  • Mazarei M, Teplova I, Hajimorad MR, Stewart CN. ۲۰۰۸. Pathogen ...
  • Mekapogu M, Jung JA, Kwon OK, Ahn MS, Song HY, ...
  • Moradyar M, Zamani MR, Motalebi M, Aghazadeh R. ۲۰۱۶ a. ...
  • Moradyar M, Motallebi M, Zamani MR, Aghazadeh R. ۲۰۱۶ b. ...
  • Neuhaus JM, Ahl-Go P, Hinz U, Flores S, Meins F. ...
  • Nürnberger T, Brunner F. ۲۰۰۲. Innate immunity in plants and ...
  • Pan H, Wei Y, Xin F, Zhou M, Zhang S. ...
  • Qing CM, Fan L, Lei Y, Bouchez D, Tourneur C, ...
  • Rushton PJ, Reinstädler A, Lipka V, Lippok B, Somssich IE. ...
  • Salinas J, Oeda K, Chua NH. ۱۹۹۲. Two G-box-related sequences ...
  • Shokouhifar F, Zamani MR, Motallebi M. ۲۰۱۱ a. Expression pattern ...
  • Shokouhifar F, Zamani MR, Motallebi M, Mousavi A, Malboobi MA. ...
  • Tiwari S, Mishra DK, Chandrasekhar K, Singh PK, Tuli R. ...
  • Venter M. ۲۰۰۷. Synthetic promoters: genetic control through cis engineering. ...
  • Zou X, Song E, Peng A, He Y, Xu L, ...
  • نمایش کامل مراجع