Use of Mesenchymal Adult Stem Cell for Cartilage Regeneration by Hydrogel

Background and Aims: Cartilage is a very specific tissue, which does not have the capacity to heal and renew itself. Although the invention of the method of surgery with autologous chondrocyte transplantation, developed tools to treat the cartilage lesions, it couldn’t gain a great success due to problems such as damage to the area of donation. Using the mesenchymal stem cells derived from adipose and culturing and differentiating them on scaffolds was considered appropriate as a successful research and clinical strategy. Materials and Methods: In the present study, the mesenchymal stem cells were separated from adipose tissue and cultured in two scaffolds of fibrin glue and alginate medium. After ۱, ۷ and ۱۴ days of cell differentiation, the survival ability of the differentiated cells were analyzed by Chondrogenic MTT. Moreover, type I and II collagen, aggrecan and Sox۹ expression were measured via real time-polymerase chain reaction. In addition, cartilage reconstruction on scaffolds was shown by a histological investigation. Results: Our results showed that the expression of CD۹۰ and CD۱۰۵ as mesenchymal markers is at a high level whereas the expression of CD۳۴ and CD۴۵ reaches a low level. The LSD test demonstrated that there was no remarkable difference among the chondrogenic MTT, scaffolds groups and control in ۷ and ۱۴ days after cell differentiation (p<۰.۰۵), although, fibrin glue had the highest expression in chondrogenic gens. Conclusions: Finding suggests that in order to utilize a new strategy for tissue regeneration utilization of inherent scaffolds such as fibrin glue can act as a protector for mesenchymal stem cells.

کلیدواژه ها:

Adipose tissue ، Alginates ، Fibrin tissue adhesive ، Mesenchymal stem cell ، سلول های بنیادی مزانشیمی ، بافت چربی ، چسب فیبرینی ، آلژینات