Comparison of PLGA/Fibrin and PLGA/Hyaluronic Acid Scaffolds for Chondrogenesis of Human Adipose-Derived Stem Cells

سال انتشار: 1399
نوع سند: مقاله ژورنالی
زبان: انگلیسی
مشاهده: 118

فایل این مقاله در 10 صفحه با فرمت PDF قابل دریافت می باشد

استخراج به نرم افزارهای پژوهشی:

لینک ثابت به این مقاله:

شناسه ملی سند علمی:

JR_JIML-7-2_007

تاریخ نمایه سازی: 6 اسفند 1401

چکیده مقاله:

Background and Aims: Tissue engineering is a relatively novel field that has been intensely developing during recent years and has shown to be excessively promising when used for cartilage regeneration. Scaffolds represent important components for tissue engineering. Materials and Methods: The Poly Lactic-Co-Glycolic Acid (PLGA) impregnated with fibrin and hyaluronic acid (HA) produce hybrid scaffolds. human adipose-derived stem cells (hADSCs) were seeded in scaffolds and cultured in chondrogenic media. The viability of cells in different groups was assessed by MTT. The expression of chondrogenic related genes [Sox۹, type II collagen (Col II), Aggrecan(AGG)] and type X collagen (Col X) was quantified by real-time polymerase chain reaction. Results: The results of the real-time PCR showed SOX۹, AGG and Col X gene expression in the control groups being significantly lower than the other groups (p≤۰.۰۵). It also demonstrated Col II gene expression in the control group being significantly lower than the PLGA/Fibrin and PLGA/Fibrin/HA groups (p≤۰.۰۵). The Col X gene expression of cells in PLGA/HA and PLGA/Fibrin/HA groups significantly decreased in comparison with the PLGA/Fibrin group (p≤۰.۰۵). Conclusions: These conclusions indicate that administration of PLGA/ Fibrin and PLGA/HA scaffolds, particularly PLGA/Fibrin/ HA, motivates chondrogenesis in hADSCs. This can be diminished by decreasing hypertrophic markers and increasing characteristic markers of hyaline cartilage.

نویسندگان

زین العابدین شریفیان

Department of Anatomy, Faculty of Medicine, Hormozgan University of Medical Sciences, Bandar Abbas, Iran

بتول هاشمی بنی

Department of Anatomical Sciences and Molecular Biology, Isfahan University of Medical Sciences, Isfahan, Iran.

مجید پورانتظاری

Department of Biology and Anatomical Sciences, Shahid Sadoughi University of Medical Sciences, Yazd, Iran.

علی ولیانی

Department of Anatomical Sciences and Molecular Biology, Isfahan University of Medical Sciences, Isfahan, Iran.

محمد مردانی

Department of Anatomical Sciences and Molecular Biology, Isfahan University of Medical Sciences, Isfahan, Iran.

محمد زمانی رارانی

Department of Anatomy, Faculty of Medicine, Hormozgan University of Medical Sciences, Bandar Abbas, Iran.

حمیدرضا پورانتظاری

Faculty of Dentistry, Shahid Sadoughi University of Medical Sciences, Yazd, Iran.

مراجع و منابع این مقاله:

لیست زیر مراجع و منابع استفاده شده در این مقاله را نمایش می دهد. این مراجع به صورت کاملا ماشینی و بر اساس هوش مصنوعی استخراج شده اند و لذا ممکن است دارای اشکالاتی باشند که به مرور زمان دقت استخراج این محتوا افزایش می یابد. مراجعی که مقالات مربوط به آنها در سیویلیکا نمایه شده و پیدا شده اند، به خود مقاله لینک شده اند :
  • . Paduszyński P, Aleksander-Konert E, Zajdel A, Wilczok A, Jelonek ...
  • . Makadia HK, Siegel SJ. Poly lactic-co-glycolic acid (PLGA) as ...
  • . Hashemibeni B, Valiani A, Esmaeli M, Kazemi M, Aliakbari ...
  • . Snyder TN, Madhavan K, Intrator M, Dregalla RC, Park ...
  • . Chung C, Burdick JA. Influence of three-dimensional hyaluronic acid ...
  • . LeBoeuf RD, Raja R, Fuller G, Weigel P. Human ...
  • . Mazini L, Rochette L, Amine M, Malka G. Regenerative ...
  • . Hashemibeni B, Razavi S, Esfandiary E, Karbasi S, Mardani ...
  • . Tavakoli E, Mehdikhani-Nahrkhalaji M, Hashemi-Beni B, Zargar-Kharazi A, Kharaziha ...
  • . Hashemibeni B, Pourentezari M, Valiani A, Zamani M, Mardani ...
  • . Mardani M, Hashemibeni B, Ansar MM, Esfahani SHZ, Kazemi ...
  • . D'andrea F, De Francesco F, Ferraro GA, Desiderio V, ...
  • . Dai W, Kawazoe N, Lin X, Dong J, Chen ...
  • . Pan Z, Ding J. Poly (lactide-co-glycolide) porous scaffolds for ...
  • . Sonomoto K, Yamaoka K, Kaneko H, Yamagata K, Sakata ...
  • . Knudson CB. Hyaluronan and CD۴۴: strategic players for cell-matrix ...
  • . Kawakami Y, Rodriguez-León J, Belmonte JCI. The role of ...
  • . Davies RL, Kuiper NJ. Regenerative medicine: a review of ...
  • . Zhu M, Feng Q, Sun Y, Li G, Bian ...
  • . Bauer C, Berger M, Baumgartner RR, Höller S, Zwickl ...
  • . Wu SC, Chang JK, Wang CK, Wang GJ, Ho ...
  • . Liu Y, Shu XZ, Prestwich GD. Osteochondral defect repair ...
  • . Feng Q, Lin S, Zhang K, Dong C, Wu ...
  • . Bian L, Guvendiren M, Mauck RL, Burdick JA. Hydrogels ...
  • . La Gatta A, Ricci G, Stellavato A, Cammarota M, ...
  • . Dragoo JL, Carlson G, McCormick F, Khan-Farooqi H, Zhu ...
  • . Girandon L, Kregar-Velikonja N, Bozikov K, Barlic A. In ...
  • . Cakmak O, Babakurban ST, Akkuzu HG, Bilgi S, Ovalı ...
  • . Lee JC, Lee SY, Min HJ, Han SA, Jang ...
  • نمایش کامل مراجع