Utilizing a Three-phase Hollow Fiber Liquid-phase Micro Extraction Method for Metoprolol Succinate Extraction from Biological Urine Prior to HPLC-UV Analysis

This study presents hollow fibers in liquid-phase micro extraction (LPME) method as a technique, in order to pre-concentrate Metoprolol Succinate drugs from tap water and urine samples. The extracted analyte with hallow fiber was detected with high performance liquid chromatography equipped with ultraviolet detector (HPLC-UV). The effect of several parameters on extraction efficiency such as organic solvent type, receiving phase, pH of receiving and extractant phase, the extraction time and mixing speed were investigated and optimized. After extracting the Metoprolol Succinate drug under the optimum conditions such as types of extraction solvents, stirring speed, extraction time and solvent pH, the pre-concentration factor was obtained ۱۹۲ for tap water and ۱۶۹ for urine samples. The obtained detection limit was ۳.۹۰ μg/L for urine and ۱.۵ μg/L for tap water in which R۲ > ۰.۹۹۵. The extraction recovery for real samples was ۹۴% for urine sample and ۹۸ % for tap water.

کلیدواژه ها:

Hollow fiber liquid phase micro extraction ، The human urine sample ، Metoprolol Succinate ، High performance liquid chromatography ، Ultraviolet detector