Neuroprotective effects of human olfactory ectomesenchymal stem cells on histological and behavioral aspects in a rat model of cerebellar ataxia

Cell replacement therapy (CRT) is one of the most effective approaches used to alleviate symptoms of neurodegenerative syndromes such as cerebellar ataxia (CA). Human olfactory epithelium mesenchymal stem cells (OE-MSCs) have been recognized as a promising candidate for CRT, due to their distinctive features including immunomodulatory properties and ease of accessible compared to other types of MSCs. Hence, the main goal of our study was to explore the impacts of OE-MSCs transplantation on behavioral, structural, and histological deficiencies in a rat model of CA. After obtained an informed consent from volunteers, OE-MSCs were obtained from their nasal cavity. Then, OE-MSCs were characterized by the positive expression of CD۷۳, CD۹۰, and CD۱۰۵ as MSCs as well as nestin and vimentin as primitive neuroectodermal stem cells markers. Then, the animals were randomized into three control, ۳-acetylpyridine (۳-AP) treated, and ۳-AP + cell groups. In both experimental groups, the rats received intraperitoneal injection of ۳-AP (۷۵ mg/kg), followed by the implantation of OE-MSCs into the cerebellum of ۳-AP + cell group. The impact of engrafted OE-MSCs on motor coordination and performance along with biochemical, immunohistochemical, and stereological changes in the cerebellum of the rat models of CA were investigated. According to our findings, the administration of ۳-AP decreased the cerebellar GSH concentration. The injection of ۳-AP also altered the morphological characteristics of the cerebellar Golgi cells. On the other hand, OE‐MSCs transplantation improved motor coordination in CA. Besides, the implantation of OE‐MSCs reduced caspase-۳ expression and microglia proliferation in the cerebellum upon ۳-AP administration. Finally, the transplant of OE‐MSCs protected Purkinje cells against ۳-AP toxicity. In sum, the present study revealed considerable advantages of OE-MSCs in managing CA animal model.

کلیدواژه ها:

Cerebellum ، Neurodegenerative disease ، Cerebellar ataxia ، ۳-AP ، Olfactory stem cells