Ceratonia siliqua L improved cryodamage of asthenozoospermic specimens: An experimental study

Abstract Background: Sperm freezing is an important procedure in assisted reproductive technology. Freezing results in physical and chemical changes in the sperm. Ceratonia siliqua L (C.siliqua) is a tree that has antioxidant properties. Objective: This study aimed to investigate the effect of different concentrations of C.siliqua in a freezing medium on semen parameters, and some biochemical parameters in asthenozoospermic specimens. Materials and Methods: ۴۰ asthenozoospermic specimens (semen specimens with motility < ۳۲%) were obtained from men aged between ۲۰-۴۰ yr according to the World Health Organization criteria. Each sample was divided into ۶ groups: I) fresh, II) control, III) ۵, IV) ۱۰, V) ۲۰, and VI) ۳۰ µg/ml C.siliqua extract were added to a freezing medium respectively. Then sperm parameters, malondialdehyde, total antioxidant capacity, reactive oxygen species, and sperm DNA assay were evaluated using related protocols after thawing. Results: Data analysis shows that sperm parameter, and total antioxidant capacity level increased at a concentration of ۲۰ µg/ml of C. siliqua extract compared to the other concentrations of C.siliqua extract after cryopreservation and thawing (p < ۰.۰۰۱). Also, the sperm DNA fragmentation assay, reactive oxygen species, and malondialdehyde levels were significantly reduced by adding ۲۰ µg/ml of C. siliqua extract to the sperm freezing medium compared to the other treated groups after cryopreservation (p < ۰.۰۰۱). Conclusion: C.siliqua extract significantly improved sperm parameters after cryopreservation and thawing in asthenozoospermic specimens, and the greatest impact was observed at the ۲۰ µg/ml C.siliqua L extract concentration (p < ۰.۰۰۱).

کلیدواژه ها:

Asthenozoospermia ، Ceratonia ، Cryopreservation ، Fertility preservation ، Infertility ، Male.Infertility.