The Effect of Amniotic Membrane Scaffold on AmnioticFluid Mesenchymal S tem Cell Growth and Their Differentiationinto Neuron Cells

Objective: Amniotic fluid mesenchymal s tem cells (AFMSCs)are isolated from AF during PGD. AFMSCs differentiate toother cell lines including neuronal cells under s tandard differ entiation conditions. Scaffold allows cells to attach, causes cellmigration, cell production, and transfer biochemical factors.AM is the part of the placenta that can be used as a natural scaffoldin research.Materials and Methods: AM was decellularized, cut intosmall pieces, and placed on a petri dish surface. AF cells weresuccessively passaged, and cultured on Petri dishes with/withoutscaffold. AFMSCs grew well and the cells were confirmedto be mesenchymal by flow cytometry. They were cultured ina differentiation medium with growth and inducing factors tos timulate nerve differentiation, which was confirmed by realtimepolymerase chain reaction (PCR). Las tly, the effect of theAF scaffold on neural differentiation of AFMSCs was assessedusing scanning electron microscopy (SEM) imaging.Results: It was observed that the cells were well adhered to thecell scaffold and their growth was better. These results wereconfirmed by SEM imaging. Generally, after cell culture on thescaffold and without the scaffold, which was performed in consecutivepassages, cell differentiation to neural cells using thescaffold showed a greater increase in differentiation than cellculture without the scaffold. ANOVA analysis also confirmedthis observation (P value <۰.۰۵).Conclusion: The findings of this s tudy can be useful in improvingthe neural differentiation of AFMSCs, which is an accessibleand valuable resource. It is also promising to use these cellsin recons tructive medicine and in the treatment of nerve tissueand degenerative diseases of nerve cells.

کلیدواژه ها:

Amniotic Fluid ، Mesenchymal S tem Cell ، Neural Differentiation ، Scaffold