Ultras tructure of Human Ovarian Tissues Transplantedto Chick Embryo Chorioallantois Membrane(CAM) after Vitrification or Slow Freezing

Background: Ovarian follicle depletion and premature ovarianfailure are significant challenges in cancer patients subjected toradio- or chemotherapy, ovarian tissue cryopreservation (OTC)is a suitable option. The aim was to analyze the s tructure andultras tructure of human ovarian tissues transplanted onto chickembryo chorioallantois membrane (CAM) after cryopreservationby vitrification or slow freezing.Materials and Methods: Ovarian tissues from ۱۰ cancer patientsunderwent cryopreservation. CAM transplantation wasdone on fresh and cryopreserved OTs, to assign samples tonine s tudy groups as follows: ۱) FI-FIII= fresh, ۵- and ۱۰-dayspos t-CAM transplantation groups; ۲) VI-VIII= vitrified, ۵- and۱۰-days pos t-transplantation vitrified groups; ۳) SFI-SFIII:slow frozen, ۵- and ۱۰-days pos t-transplantation slow freezinggroups. Proliferation ability, folliculogenesis, s tructural and ultrastructure were analyzed.Results: The density of primordial follicles did not change afterboth freezing methods but reduced after ۵ (P≥۰.۰۵) and ۱۰days (P≤۰.۰۵) pos t-CAM transplantation. The follicular gradesignificantly decreased in all transplanted tissues (P≤۰.۰۵).Proliferation marker increased after cryopreservation but wasreduced after transplantation (P≤۰.۰۵). The ultras tructure ofovarian follicles was more preserved in the fresh groups. S tromalultras tructure appearing more preserved after vitrificationcompared with slow freezing. The results showed no any signof infection by malignant cells after transplantation.Conclusion: TEM evaluation showed follicular damages inboth methods of freezing, with a better transplantation rate aftervitrification. Also, enhanced follicular activation and depletionwere observed after vitrification. Pos t grafting evaluation of theearly events of OTs transplantation may improve the long termmaintenance of OTs s torage.

کلیدواژه ها:

Cancer Patients ، Ovarian Tissue Cryopreservation ، Tem Evaluation ، Slow Freezing ، Vitrification