The Role of Rosmarinic Acid Loaded to Serum-derivedExosome as A Factor to Resucing Inflammation inMice with Induced Endometritis

Background: The aim of this s tudy was to evaluated the role ofRormarinic acid loaded to exosome to reducing uterine inflammationin mice with induced endometritis with LPS.Materials and Methods: For this purpose, Balb-C mice serumwas firs t obtained and then exosome derived from serum byultracentrifugation technique. Then, using sonication method,۵ and ۱۰ mg doses of Rosmarinic acid were loaded into theexosomes. After evaluating the quality and amount of drugloading sys tem, in ۶ experimental groups, the mice induced endometritiswith LPS. They were treated (control-inflammation-Romarinic acid-exosome-drug + exosome-drug loaded in theexosome). Then, the incidence of inflammation and the effectivenessof the drug in uterine tissue were evaluated by his tologicalanalysis, and the expression of inflammatory genes suchas, NLRP۳, IL-۱۸ and TNF-αwas measured by qPCR.Results: The results showed that pathological lesions, particularlyinflammatory cell infiltration and expression of inflammationrelated genes attenuated in all treatments, which were moreremarkable in the higher dose groups (۱۰ mg/kg) (P˂۰.۰۵).Importunately, the bes t improvement effect was found in theRLE۱۰-group. In comparison, the lowes t treated impact wasobserved in the Exo۵ and R۵ groups.Conclusion: As a result, in the high doses of Rosmarinic acidgroups with exosome and loading of this drug with exosome,they could have a better effect in reducing inflammation in micewith endometritis.

کلیدواژه ها:

Drug-loading ، Inflammation ، qPCR ، RosmarinicAcid ، Sonication