Evaluation of Sperm Cryopreservation Using DeepEutectic Solvents on Gene Expression of Spaca۳ and SpermQuality

Background: It is possible to reduce the fertility potential ofthe individual, which can use the technique of sperm freezingto preserve male fertility potential. In this way, the aim of thiss tudy was to inves tigate the effects of sperm cryopreservationin the presence of Deep Eutectic Solvents (DESs) on the expressionof sperm gene such as SPACA۳ (The protein from thisgene plays an important role in the binding of sperm to the oocyte.)and its relationship with semen quality and parameters.Materials and Methods: In this s tudy, normozoospermic sampleswere collected from Mehr IVF center (Rasht, Iran). Thesemen samples were grouped as following: I. control (nonfreeze),II. freezed with SpermFreezeTM (Fertipro Co.), III.freezed with DES (EtP), and IV. freezed with DES (GlyP). Afterthawing process of samples, RNA extraction was performed forevaluation SPACA۳ gene expression by reverse transcriptionquantitativePCR. In addition, chromatin s tructure, chromatincondensation, viability, and acrosome reaction of sperm weredetermined for each group.Results: The results show that cryopreservation using DEScould preserve the semen quality (such as chromatin integrityand condensation, and intact acrosome) and sperm parameters(such as viability, motility, morphology, concentration). So that,there is not significant difference compared to I (P>۰.۰۰۵) andII groups (P>۰.۰۵). It has also shown that the presence of thesesolvents did not alter the expression quality of sperm genessuch as SPACA۳ in normozoospermia samples.Conclusion: This s tudy shows that DES solvents were introducedas high performance sperm cryopreservation.

کلیدواژه ها:

DES ، Male Infertility ، SPACA۳ ، Sperm Freezing ، Vitrification