Ameliorative Effects of Sildenafil Citrate on SpermBiochemical Parameters during Cryopreservation in TheAs thenozoospermic Men
محل انتشار: بیست و سومین کنگره بین المللی هیبریدی پزشکی تولید مثل و هجدهمین کنگره هیبریدی فناوری سلولهای بنیادی رویان
سال انتشار: 1401
نوع سند: مقاله کنفرانسی
زبان: انگلیسی
مشاهده: 97
نسخه کامل این مقاله ارائه نشده است و در دسترس نمی باشد
- صدور گواهی نمایه سازی
- من نویسنده این مقاله هستم
استخراج به نرم افزارهای پژوهشی:
شناسه ملی سند علمی:
RROYAN23_067
تاریخ نمایه سازی: 17 دی 1401
چکیده مقاله:
Background: As thenozoospermia reduces sperm motility resultingin infertility. Reactive oxygen species (ROS), producedduring cryopreservation, impair sperm function. The effect ofSildenafil Citrate (SC) supplementation on oxidative s tress parametersduring cryopreservation of semen samples of As thenozoospermiamen was assessed.Materials and Methods: ۳۰ semen samples were collectedfrom As thenozoospermic patients. Each sample was dividedinto three groups: Control (fresh), Freeze (treated with cryoprotectant),and Freeze+SC (treated with cryo-protectant+۰.۶۷μM SC solution). In each sample, the level of sperm Tumornecrosis factor alpha (TNF-α), Malondialdehyde (MDA)and sperm antioxidant enzymes, including Catalase (CAT),Glutathione (GSH), and Superoxide dismutase (SOD) wereanalyzed using Enzyme-linked immunosorbent assay (ELISA).Results: Our results showed a significant reduction in the levelof sperm antioxidant enzymes and also a significant increase insperm TNF-α and MDA level in the freeze group compared tothe control group (P<۰.۰۵). whereas in the Freeze+SC group,a significant increase in sperm antioxidant enzymes level anda significant decrease in sperm TNF-α and MDA level was observedcompared to the freeze group (P<۰.۰۵).Conclusion: SC supplementation in cryopreserved sperms ofas thenozoospermic men, leads to a major improvement of pos tthawsperm quality and sperm biochemical parameters throughreducing ROS and preventing the undesired effects of ROS onsperm function.
کلیدواژه ها:
نویسندگان
R Kohzadi
Biology Department, University of Arak, Arak, Iran
M Soleimani Mehranjani
Biology Department, University of Arak, Arak, Iran
MA Shariatzadeh
Biology Department, University of Arak, Arak, Iran
E Cheraghi
Biology Department,, University of Qom, Qom, Iran