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Expressed Cellobiohydrolase Enzyme of Thermobifidia fusca in Pichia pastoris as Host Can Act on Cotton Substrate

سال انتشار: 1401
نوع سند: مقاله ژورنالی
زبان: انگلیسی
مشاهده: 242

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شناسه ملی سند علمی:

JR_JCMR-14-1_001

تاریخ نمایه سازی: 17 آبان 1401

چکیده مقاله Expressed Cellobiohydrolase Enzyme of Thermobifidia fusca in Pichia pastoris as Host Can Act on Cotton Substrate

Cellulose which is extremely produced by plants, can be used for biofuel production but this function needs chemical or enzymatic digestion. Cellulose hydrolysis of plant wastes for ethanol production requires a mixture of three enzyme groups, including endoglucanases, exoglucanases, and beta-glucosidases. The cellobiohydrolase enzyme (Cel6B) from Thermobifidia fusca has been used for cellulase activity extensively. This research aimed to express recombinant Cel6B enzyme in Pichia pastoris. For this purpose, cel6B gene in control of AOX1 promoter (methanol inducible) was introduced into Pichia pastoris. Amplification of cel6B gene was performed by PCR technique and then introduced into the Phil-S1 yeast vector. The recombinant construct contained the cel6B gene sequence and PHO1 signal sequence as secretion signal was transferred into Pichia pastoris GS115 strain. The transformed yeast cells expressed the recombinant Cel6B to yield 2.104 U (µmol/min)/ml of culture medium. Purified recombinant enzyme showed the best activity at 60 °C and pH 4.5 and this was agreed with optimum conditions for recombinant Cel6B enzymes which were produced in other systems. The purity of the enzyme was examined by SDS–PAGE technique, and a single band with a molecular weight about 59.6 kDa was observed. As cel6B gene sequence was not optimized for expression in the Pichia pastoris yeast, this could be one of the reasons for low level activity of recombinant Cel6B enzyme. This thermostable enzyme can be used for cellulolytic digestion of biomaterials in biofuel production research and other uses.

کلیدواژه های Expressed Cellobiohydrolase Enzyme of Thermobifidia fusca in Pichia pastoris as Host Can Act on Cotton Substrate:

نویسندگان مقاله Expressed Cellobiohydrolase Enzyme of Thermobifidia fusca in Pichia pastoris as Host Can Act on Cotton Substrate

Karim Imangholiloo

Agricultural Biotechnology, Ferdowsi University of Mashhad, Mashhad, Iran

Nasrin Moshtaghi

Biotechnology and Plant Breeding Department, Ferdowsi University of Mashhad, Mashhad, Iran

Seyed Hasan Marashi

Biotechnology and Plant Breeding Department, Ferdowsi University of Mashhad, Mashhad, Iran

Abdolreza Bagheri

Biotechnology and Plant Breeding Department, Ferdowsi University of Mashhad, Mashhad, Iran

Ahmad Sharifi

Ornamental Biotechnology Department, ACECR, Mashhad Branch, Mashhad, Iran

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Abd Rahman R. N. Z., Geok L. P., Basri M. ...
Fang H. and Xia L. (۲۰۱۵) Heterologous expression and production ...
Fang H., Zhao R., Li C. and Zhao C. (۲۰۱۹) ...
Gomez del Pulgar E.M. and Saadeddin A. (۲۰۱۴) The cellulolytic ...
Harshvardhan K., Mishr A. and Jha B. (۲۰۱۳). Purification and ...
Kazzaz A. E. and Fatehi P. (۲۰۲۰) Technical lignin and ...
Laemmli U. K. (۱۹۷۰). Cleavage of structural proteins during the ...
Lindenmuth B. E. and McDonald K. A. (۲۰۱۱) Production and ...
Murao S., Sakamoto R. and Arai M. (۱۹۸۸) Cellulase of ...
Lu W., Li D. and Wu Y. (۲۰۰۳) Influence of ...
Rai P., Tiwari S. and dan Gaur R. (۲۰۱۲). Optimization ...
Srivastava N., Srivastava M., Mishra P. K., Gupta V. K., ...
Sun F. F., Bai R., Yang H., Wang F., He ...
Sun Y. and Cheng J. (۲۰۰۲) Hydrolysis of lignocellulosic materials ...
Taha, A. S. J., Taha, A. J., & Faisal, Z. ...
Taha, A. S. J., Taha, A. J. and Faisal, Z. ...
Tenkanen M., Vršanská M., Siika-aho M., Wong D. W., Puchart ...
Volossiouk T., Robb E. J. and Nazar R. N. (۱۹۹۵) ...
Vuong T. V. and Wilson D. B. (۲۰۰۹). Processivity, synergism, ...
Vuong T. V. and Wilson D. B. (۲۰۰۹). The absence ...
Wu M., Bu L., Vuong T.V., Wilson D.B., Crowley M.F., ...
Xiao W., Li H., Xia W., Yang Y., Hu P., ...
Zhang N., Wright T., Wang X., Savary B. J. and ...
Zhang P., Yuan X., Du Y. and Li J. J. ...
Zhang Y.P., Hong J. and Ye X. (۲۰۰۹). Cellulase assays. ...
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