Importance of QF-PCR method in aborted embryos in comparison with other common relative determination aneuploidies methods

One of the most important method in cytogenetic in order to diagnose the chromosomal abnormalities, is QF-PCR (Quantitative fluorescent polymerase chain reaction).In this way, QF-PCR can be employed in diagnosing the chromosome duplication number by amplification of repeat sequences at polymorphic loci. These repeat sequences are amplified by PCR, and the labelled yields are classified by gel electrophoresis method. Importantly, QF-PCR reaction has been in diagnostic application in many countries and has confirmed to be a robust, cost-effective, and precise rapid prenatal test for many types of common aneuploidies. Special benefits comprise detection of mosaicism, triploidy and maternal cell contamination. So, we try to declare the importance of this technique in comparison with other ones in this review article.

کلیدواژه ها:

QF-PCR ، Aneuploidies ، Abortion ، Chromosomal abnormalities