Molecular phylogeny of three desert truffles from Iran based on ribosomal genome

The ITS region including the ۵.۸S gene of rDNA of three desert truffle species were amplified using ITS۴ and ITS۱ primers. The ITS sequences were compared to those of other related authentic sequences obtained from GenBank. Among ۱۲ specimens studied, seven isolates corresponded to Terfezia claveryi reported by other authors. Iranian T. claveryi specimens had an average similarity of ۹۹.۴% (range ۹۸.۷–۱۰۰%) among themselves, while all T. claveryi sequences analyzed had an average of ۹۵.۲% (range ۸۷.۲–۱۰۰%) similarity. Four specimens corresponded to Trimania pinoyi, being a sister taxon to Tirmania nivea, of whichonly one specimen could be studied. Iranian T.pinoyi specimenshad an average of ۹۹.۹% similarity (range ۹۹.۸–۱۰۰%) among themselves, and ۹۷.۲% (range ۹۳.۱– ۱۰۰%) between all T. pinoyi sequences compared. 

کلیدواژه ها:

Ascomycota ، Phylogenetics ، Ectomycorrhizal fungi ، internal transcribed spacer of rDNA