Study of genetic variation among some Gossypium spp. genotypes available in Iran using ISSR molecular marker

The current study was conducted to explore genetic variations among several cotton species, including Gossypium hirsutum cultivar Varamin, G. barbadense cultivar T۱۴, G. herbaceum cultivar Arya, hybrid of G. hirsutum cultivar Varamin × G. barbadense cultivar T۱۴ (V×T۱۴), and a hybrid of G. hirsutum cultivar Varamin × G. herbaceum cultivar Arya (V×A) using inter-simple sequence repeats (ISSR). In this study, a total of ۶۰ bands were amplified using four ISSR marker primers. As a result, ۵۷ bands showed polymorphism. The results showed a high level of diversity in the studied varieties. The highest percentage of polymorphism was related to ISSR۴ primer with ACACACACACACACACT sequence (۱۰۰%) and the lowest percentage was related to ISSR۱ primer with AGAGAGAGAGAGAGAGC sequence (۹۲.۸%). Cluster analysis showed three major groups. The first group consisted of Arya and Varamin, the second group was comprised of V×A, T۱۴ which showed a high genetic similarity indicating T۱۴ originates from Varamin cultivar, and the third group contained V×T۱۴. Overall, the results showed that, ISSR markers can be effectively used to study the genetic diversity of cotton.  

کلیدواژه ها:

Cotton variations ، cultivar ، diploid ، Genetic diversity ، Malvaceae ، Tetraploid