Research Article: Histological effects of water-soluble fraction of diesel (WSFD) on liver, gill, and kidney of common carp (Cyprinus carpio)

This research aimed to study the histological effects of the acute water-soluble fraction of diesel (WSFD) on the liver, gill, and kidney of common carp (Cyprinus carpio). The fish were divided into four experimental groups; control (group۱) (G۱) without WSFD, and three groups of WSFD with densities of ۴% (G۲), ۸% (G۳), and ۱۶% (G۴). After ۴۸ hours of exposure, sampling of liver, gill, and kidney was performed from all experimental groups, simultaneously. The results showed that the main alterations observed in the gill included goblet cell increasing, epithelial lifting, complete and incomplete fusion, filamentous edema, blood congestion, aneurysm, and infiltration of inflammatory cells causing the rank of gill tissue lesions in the G۴ (۳.۳۰) and G۳ (۲.۷۰) significantly differed from the G۱(۰.۰۰) (p<۰.۰۰۵). Sever sinusoid dilation, sever blood congestion, hypertrophy a of nucleus, nucleus in lateral position, cytoplasmic vacuolization and pyknotic nucleus were observed in the liver. The rank of liver tissue lesions in the G۴ (۳.۳۰) and G۳ (۲.۷۰) significantly differed from the G۱(۰.۰۰, p<۰.۰۰۵). Tubular disorganization, shrinkage and necrosis of tubule, and melanomacrophage aggregation were observed in the kidney. The rank of kidney tissue lesions in the G۴ (۳.۳۰) significantly differed compared to the G۱ and G۲ (۰.۰۰) (p<۰.۰۰۵). Total lesion rank of whole aforementioned tissues in G۴ (۹.۷۰) significantly differed from the G۱ (۰.۰۰) and G۲ (۳.۷۰) (p<۰.۰۵). The results showed that WSFD causes pathological lesions incidence in the fish liver, gill, and kidney and increase in WSFD level causes more severe tissue damages.

کلیدواژه ها:

Water-soluble fraction of diesel (WSFD) ، Histopathology ، Gill ، Liver ، Kidney ، Common Carp.