Molecular identification of bacteria isolated from vaginalinfections of infertile women

Background and Aim : Infertility is one of the most critical problems of human societies, whichhas a significant percentage all around the world. Infertility occurs due to a disorder in thereproductive system, which is defined as the inability of couples to bear children after at least ۱۲months of consecutive unprotected intercourse. There are many factors involved in infertility thatcause defects in reproductive performance in men or women. It has been reported that femalegenital infections are possible causes of female infertility. In this study, bacteria causing vaginalinfection in infertile women were identified by molecular method.Methods : After sampling from the infectious vagina of infertile women, the samples werecultured in suitable bacterial culture media, such as Brain Heart Agar. Bacteria were purified, andPCR products using universal primers were sequenced. In order to identify the isolated bacteria,the obtained sequences were analyzed using bioinformatics methods.Results : The results of bioinformatics analysis showed that Escherichia coli is the dominantbacterium causing infection in the vagina of infertile women. Also, Enterococcus was anotherbacterium that was isolated from the infected vagina of some women.Conclusion : Bacterial infections of the genital tract of infertile women should be considered andtreated as a possible cause of infertility.

کلیدواژه ها:

infertility ، bacteria ، vaginal infection