Studu on Antibacterial activity of extracted phycocyanin fromspirulina platensis

Background and Aim : Phycocyanin, a blue color pigment is a common pigment ofcyanobacteria. Phycocyanin is used as colorant in food and cosmetics. It was known as antioxidantand has some therapeutic value such as immuno-modulating activity, anticancer activity andantimicrobial activity.Methods : The phycocyanin of cyanobacterium, Spirulina platensis, was extracted by using twophysicochemical methods and purified by ammonium sulfate precipitation and dialysis. Theconcentration and extraction yield of phycocyanin was calculated. Antibacterial assay wereapplied for against ۶ type test strains consist of gram positive and gram negative bacteria. Activitywas determined by using direct drop and agar well diffusion methods.Results : It was found that the phycocyanin concentration was ۲mg/mL and Extraction yield wasobtained to be ۴mg/g. The protein estimation of the phycocyanin pigment using Lowry methodwas found to be ۱۲۸ μg/ml. Each extracts exhibited varying degrees of antibacterial activity againstan array of all Gram-positive bacteria but no effect against Gram-negative bacteria was showed.Conclusion : The results of this study indicate the potential use and ability of Spirulina platensisand extracted phycocyanin as a source of antimicrobial agent against some bacteria.

کلیدواژه ها:

Spirulina platensis ، Phycocyanin extraction ، Antibacterial activity