In vitro Qualitative Phytochemical Analysis and AntibacterialActivity of Ethanolic Extract of Flowers of Rheum ribes L. fromTonekabon - Iran

Background and Aim : Plants are an important source for the discovery of new products ofmedicinal value for drug development and plants secondary metabolites are unique sources forpharmaceuticals food additives, flavors, and other industrial values. The aim of this project was toevaluate the phytochemical activity of ethanolic extract of flowers of Rheum ribes L. belonging tothe family Polygonaceae from Tonekabon, Iran.Methods : In this project, the flowers were collected and extract prepared from ethanol bymicrowave assisted extraction (MAE) method. The present study revealed that the phytochemicalsanalysis of nine different chemical compounds terpenoids (Salkowski Test), flavonoids (AlkalineReagent Test), phenols (Ferric Chloride Test), coumarins (sodium hydroxide Test), tannins (FerricChloride Test), phlobatannins (HCl Test), cardiac glycosides (Keller-Killani test), quinones(H۲SO۴ Test), and saponins (Foam Test) were tested in ethanolic extracts. Later, the antibacterialactivity of the ethanolic extract of flowers of Rheum ribes L. was tested using both gram positiveas well as gram negative bacteria i.e. (Bacillus subtilis, Staphylococcus aureus, Pseudomonasaeruginosa and Escherichia coli) respectively using minimum inhibitory concentration (MIC) andminimum bactericidal concentration (MBC) methods.Results : The results of the phytochemical screening of ethanolic extract of flowers of Rheumribes L. were (terpenoids, flavonoids, phenols, coumarins, quinones and tannins) presented. Theethanolic extract of flowers of Rheum ribes L. exhibited good antibacterial potential against grampositive and gram negative bacterial strains.Conclusion : Conclusively, Rheum ribes L. is valuable source for new compounds and shouldreceive special attention in research strategies to develop new antibacterial urgently required in thenear future.

کلیدواژه ها:

Rheum ribes L. ، Bacillus subtilis ، MIC ، Flavonoids