Background and Aim : The expansion of human industrial activities in the present age has led toa significant increase in the amount of heavy metals in air, water, and soil. One of the major waysof entry of heavy metals into the human body is through food chain, fruits and vegetablescontamination. Probiotic Lactic acid bacteria are shown to possess the ability to aggregate heavymetals and thus represent a promising approach to decontaminating heavy metals in food andwater, and possibly the gastrointestinal tract. The aim of this investigation is to select probioticsstrain to reduce the contamination of heavy metals especially for in fruits and vegetables.Methods : Effectiveness of several LAB isolates procured from Razi vaccine and Serum ResearchInstitute, were evaluated for their ability to bind cadmium (Cd), Lead (Pb) and nickel (Ni) in invitroconditions. The parameters were studied under the conditions of constant pH, temperature,and contact time. All results were analyzed statistically.Results : Seven LAB strains with probiotic properties (L. casei, L. rhamnosus, L. plantarum, L.fermentum, Ent. faecium, L. helveticus and L. acidofilous) showed different level of bindingability to the tested heavy metals. Among the isolates, L. plantarum, L. fermentum, Ent. faeciumshowed the highest bioabsorption efficiency and were able to adsorp Pb and Cd (۹۹%) during ۱۵minutes of initial contact. However, in the case of Ni, highest biosorption rate (۹۱.۱۵±۰.۰۱%) wasseen within ۳۰ minutes of contact time. Overall, E.faecium showed highest adsorption of the testedheavy metals which were equivalent to ۷۹.۷۵±۰.۱۱, ۷۵.۲۸±۰.۰۵ and ۸۳.۹۹±۰.۱۰% for Pb, Cd andNi, respectively.Conclusion : probiotic strains (L. plantarum, L. fermentum and E.faecium) might be a potentnatural bio-sorbent for the removal of Cd, Pb and Ni removal from fruits and vegetables. However,further insitu studies are required to confirm their efficacy and applications for future use.