Synthesis silver nanoparticles using Peucedanum officinaleextract (PO@AgNPs) and investigation antibacterial and antioxidantactivities

Background and Aim : Introduction and aims: Nowadays, the biosynthesis of metallicnanoparticles is on a sharp rise because of its potential in eliminating antibiotic-resistant bacteria.So that, this study reports an eco-friendly and cost-effective methodology for synthesizingbiogenic silver nanoparticles (AgNPs) using alcoholic extract of Peucedanum officinale(PO@AgNP).Methods : Material & Methods: In this research, we used Peucedanum officinale for the greensynthesis of silver nanoparticles. In this study, various parameters such as concentration, time andtemperature were investigated to achieve optimal conditions to synthesis PO@AgNP. All stagesof reaction were monitored by UV-Vis spectroscopy. The PO@AgNPs were characterized in termsof structural properties and morphology with X-ray diffraction (XRD) and Fourier transforminfrared spectroscopy (FT-IR) and transmission electron microscopy (TEM) techniques. The brothmicrodilution method was performed to determine the antibacterial activity of PO@AgNPs. Theantioxidant activities of PO@AgNPs were examined as a percentage of DPPH inhibition.Results : Results: Examination of the XRD data confirmed the synthesis of crystalline particles.The FT-IR spectra showed that phenolic compounds in Peucedanum officinale extract act as areducing agent of silver ions and formation PO@AgNPs. TEM images showed that PO@AgNPshad a spherical morphology with a size of about ۳۰-۴۰ nm. PO@AgNPs shown strong antibacterialactivity against Klebsiella pneumoniae ,Enterococcus faecalis and Streptococcus mitis withminimum inhibitory concentrations (MIC) values were ۳۱.۲, ۶۲.۵ and ۶۲.۵ μg/ml, respectively.DPPH test have shown that with increasing the concentration of PO@AgNPs (۶۲.۵ to ۵۰۰ μg/ml),the percentage of radical inhibition of DPPH increased (۲۵% to ۵۵%).Conclusion : Conclusion: All investigations showed that PO@AgNPs have high antibacterialactivity against gram-positive and negative bacteria and have strong antioxidant properties andintroduces it as a very efficient, eco-friendly and cost-effective nanoparticle.

کلیدواژه ها:

Silver nanoparticles ، Green synthesis ، Peucedanum officinale ، Antibacterial ، Antioxidants.