New protein and bacteriophage immunoassay techniques fordetection Brucella
محل انتشار: بیست و سومین کنگره بین المللی میکروب شناسی ایران
سال انتشار: 1401
نوع سند: مقاله کنفرانسی
زبان: انگلیسی
مشاهده: 134
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شناسه ملی سند علمی:
MEDISM23_413
تاریخ نمایه سازی: 16 مهر 1401
چکیده مقاله:
Background and Aim : Brucellosis is the most zoonotic diseases caused by the Gram-negativebacterium Brucella genus that are affecting livestock and humans. The diagnosis assessments ofhuman brucellosis are considered for managing disease. The aim of this study was to bring forwarddiagnosis techniques of brucellosis.Methods : The novel protein immunoassay and detection based on bacteriophage techniques fordiagnosis of brucellosis were explored in databases such as Web of Sciences, Google Scholar,Scopus and PubMed by using index words Brucella, human brucellosis, detection, diagnosis,Serology, protein and bacteriophage between February ۲۰۱۷ and January ۲۰۲۲.Results : Many reports demonstrate that novel protein immunoassay and bacteriophage-detectingtechniques can identify brucellosis in high sensitivity and specificity in comparison with RoseBengal test (RBT), complement fixation test (CFT). These techniques were based on Brucellaspecies lipopolysaccharide or outer membrane proteins as a diagnostic antigen that was performedon serum samples.Conclusion : The result of this study indicated that protein and bacteriophage immunoassays arerapid, cost effective, highly specific and sensitive methods used for detecting Brucellosis rapidlyand can be potentially used as reliable techniques because of potential usefulness rapid detectingand identifying method and do not require complex equipments.
کلیدواژه ها:
نویسندگان
Fateme RafieiAtani
Department of Microbiology, Faculty of Medicine, Shahed University, Tehran, Iran
Zahra Rafiei Atani
Department of Microbiology, Faculty of Medicine, Shahed University, Tehran, Iran
Mohammadmehdi Ranjbar
Razi Vaccine and Serum Research Institute, Agricultural Research, Education and Extension Organization, Karaj, Iran.
Saeed Alamian
Department of Brucellosis, Razi Vaccine and Serum Research Institute (RVSRI); Agricultural Research, Education and Extension Organization (AREEO), Karaj, Iran
Mohammad Niakan
Department of Microbiology, Faculty of Medicine, Shahed University, Tehran, Iran