Identity Confirmation of IRIBA vaccine by multiplex PCRAssay and phage typing

Background and Aim : Brucellosis is a zoonotic bacterial infection caused by Brucella spp. inIran which causes great direct and indirect economic losses to the livestock industry. The effectivevaccine against bovine brucellosis is the Brucella abortus strain IRIBA vaccine which was used incattle in Iran. The aim of this work was to identify and distinguish the IRIBA vaccine by phagetyping and multiplex PCR techniques.Methods : For this purpose, two multiplex PCR through the Bruce-ladder and wboA-based PCRwere used to identify the IRIBA vaccine. Evaluation of the IRIBA vaccine also was performedthrough the classical phage typing according to the OIE protocol.Results : The IRIBA identification through the molecular methods showed as the sensitiveapproaches for detection of IRIBA vaccine strain from B. abortus filed vaccine. Bruce-ladder PCRwith ۱۶ primers showed that IRIBA and ۵۴۴ have three common PCR bands, ۴۵۰, ۵۷۸, and ۷۹۴,but the vaccine strain IRIBA has amplified a ۲۵۲۴ band instead of ۱۶۸۲ in the reference ۵۴۴. ThewboA-based PCR with three primers was amplified (۴۰۰ bp from field B. abortus and ۹۰۰ and۱,۳۰۰ bp from IRIBA). Strain IRIBA was biotyped as a typical rough B. abortus biovar ۱ and wasresistant to rifampin and penicillin. There is no agglutination with A or M antiserum. However,IRIBA is agglutinated with acriflavine.Conclusion : Distinguishing IRIBA vaccine strains from field Brucella abortus strains that causeinfections among vaccinated cattle herds in the field is essential. This study showed the PCR-basedassay to identify the IRIBA vaccine.

کلیدواژه ها:

RB۵۱ ، vaccine ، brucellosis ، cow ، IRIBA